{"title":"CRISPR \u0026 Genome Editing Enzymes","description":"\u003cp\u003eCleavion CRISPR \u0026amp; Genome Editing Enzymes focuses on programmable nuclease products for genome editing and molecular biology research. The collection includes Cas-family enzymes and related variants used for targeted DNA manipulation.\u003c\/p\u003e\u003cp\u003eShopify filters should allow customers to separate Cas9 and Cas12 products, choose NLS-tagged formats when relevant and compare pack sizes. This category should remain distinct from general restriction enzymes because customers search by genome-editing application rather than digestion workflow.\u003c\/p\u003e","products":[{"product_id":"spcas9-nls","title":"SpCas9-NLS","description":"SpCas9-NLS is an RNA-guided endonuclease from Streptococcus pyogenes. Guided by crRNA and tracrRNA (or a sgRNA formed by their fusion), SpCas9-NLS recognizes the region immediately upstream of the PAM sequence (5'-NGG) in double-stranded DNA targets and produces a double-stranded break precisely three nucleotides upstream of the PAM. SpCas9-NLS is commonly employed for gene editing. To enhance cellular editing efficiency, SpCas9-NLS contains a nuclear localization sequence (NLS) derived from Simian virus 40 (SV40) T antigen on N-termini. Additionally, this product can be utilized for the cleavage of target DNA in vitro and gene cloning, etc.","brand":"Cleavion","offers":[{"title":"100 pmol","offer_id":57188440670583,"sku":"CLV-0191","price":74.64,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0191-0-cleavion-product.png?v=1781696383"},{"product_id":"spcas9-nls-1","title":"SpCas9-NLS","description":"SpCas9-NLS is an RNA-guided endonuclease from Streptococcus pyogenes. Guided by crRNA and tracrRNA (or a sgRNA formed by their fusion), SpCas9-NLS recognizes the region immediately upstream of the PAM sequence (5'-NGG) in double-stranded DNA targets and produces a double-stranded break precisely three nucleotides upstream of the PAM. SpCas9-NLS is commonly employed for gene editing. To enhance cellular editing efficiency, SpCas9-NLS contains a nuclear localization sequence (NLS) derived from Simian virus 40 (SV40) T antigen on N-termini. Additionally, this product can be utilized for the cleavage of target DNA in vitro and gene cloning, etc.","brand":"Cleavion","offers":[{"title":"1000 pmol","offer_id":57188440834423,"sku":"CLV-0192","price":252.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0192-0-cleavion-product.png?v=1781696384"},{"product_id":"aapcas12b-crispr-nuclease","title":"AapCas12b CRISPR Nuclease","description":"AapCas12b is a Type V Cas nuclease, exhibiting good activity across 37~60°C. Guided by crRNA and tracrRNA (or sgRNA), it exhibits cis-cleavage activity against both double-stranded and single-stranded DNA targets. For double-stranded DNA targets, AapCas12b recognizes the site complementary to the crRNA (or sgRNA) spacer sequence downstream of the PAM sequence (5'-TTN) in target DNA, specifically cleaves the target double-stranded DNA to generate sticky ends. For single-stranded DNA targets, its specific cleavage is PAM-independent. After AapCas12b, target DNA and sgRNA form a ternary complex, the enzyme exhibits trans-cleavage activity, namely non-specific cleavage of single-stranded DNA with any sequence. AapCas12b exhibits good activity in common isothermal amplification buffers, making it suitable for rapid nucleic acid detection.","brand":"Cleavion","offers":[{"title":"100 pmol","offer_id":57188442734967,"sku":"CLV-0282","price":137.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0282-0-cleavion-product.png?v=1781696415"},{"product_id":"aapcas12b-crispr-nuclease-1","title":"AapCas12b CRISPR Nuclease","description":"AapCas12b is a Type V Cas nuclease, exhibiting good activity across 37~60°C. Guided by crRNA and tracrRNA (or sgRNA), it exhibits cis-cleavage activity against both double-stranded and single-stranded DNA targets. For double-stranded DNA targets, AapCas12b recognizes the site complementary to the crRNA (or sgRNA) spacer sequence downstream of the PAM sequence (5'-TTN) in target DNA, specifically cleaves the target double-stranded DNA to generate sticky ends. For single-stranded DNA targets, its specific cleavage is PAM-independent. After AapCas12b, target DNA and sgRNA form a ternary complex, the enzyme exhibits trans-cleavage activity, namely non-specific cleavage of single-stranded DNA with any sequence. AapCas12b exhibits good activity in common isothermal amplification buffers, making it suitable for rapid nucleic acid detection.","brand":"Cleavion","offers":[{"title":"1000 pmol","offer_id":57188442898807,"sku":"CLV-0283","price":617.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0283-0-cleavion-product.png?v=1781696415"}],"url":"https:\/\/cleavion.com\/collections\/crispr-genome-editing-enzymes.oembed","provider":"Cleavion","version":"1.0","type":"link"}