{"title":"DNA\/RNA Ligases \u0026 Modifying Enzymes","description":"\u003cp\u003eCleavion Ligases \u0026amp; DNA\/RNA Modifying Enzymes brings together enzymes used to join, modify, prepare or process nucleic acids. The collection includes ligases, phosphatases, kinases and related enzymes used in cloning, library preparation and molecular biology workflows.\u003c\/p\u003e\u003cp\u003eCustomers should be able to filter this category by reaction type, substrate, enzyme family and pack size. It can also overlap with cloning or DNA assembly pages when a product has more than one practical use.\u003c\/p\u003e","products":[{"product_id":"t4-dna-ligase-fast","title":"T4 DNA Ligase (Fast)","description":"T4 DNA Ligase (Fast) is produced by E.coli carrying T4 bacteriophage gene 30. The enzyme catalyzes the formation of phosphodiester bonds between 5'-phosphate groups and 3'-hydroxyl groups of double-stranded DNA or RNA. It can repair single-strand nicks in double-stranded DNA, RNA, or DNA\/RNA hybrids and can ligate DNA fragments with sticky or blunt ends. It is not active on single-stranded nucleic acids. The enzyme is primarily used for cloning of restriction endonuclease-digested DNA fragments, site-directed mutagenesis, cloning of PCR products, linear DNA circularization, and repair of double-stranded DNA nicks. T4 DNA Ligase (Fast) requires ATP as a cofactor and completes sticky end ligation reactions at room temperature in just 10 minutes.","brand":"Cleavion","offers":[{"title":"1000 U","offer_id":57188437655927,"sku":"CLV-0162","price":81.74,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0162-0-cleavion-product.png?v=1781696377"},{"product_id":"alkaline-phosphatase-fast","title":"Alkaline Phosphatase (Fast)","description":"Alkaline Phosphatase (Fast) is an enzyme that catalyzes the hydrolysis of the 5' and 3' phosphate groups of DNA, RNA, and nucleotides. It can also remove protein phosphate groups but does not hydrolyze phosphodiester and phosphotriester bonds. This enzyme can dephosphorylate the ends of all types of DNA in 10 minutes at 37°C . It has 100% activity in CutOneTM buffer and is inactivated by incubation at 80 °C for 20 minutes. When used together with LightiNingTM restriction endonucleases, it simplifies the \"digestion-dephosphorylation\" reaction in one tube.","brand":"Cleavion","offers":[{"title":"1000 U","offer_id":57188437852535,"sku":"CLV-0163","price":87.26,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0163-0-cleavion-product.png?v=1781696378"},{"product_id":"taq-dna-ligase","title":"Taq DNA Ligase","description":"Taq DNA Ligase is a thermostable ligase that catalyzes the formation of phosphodiester bonds between the 5'-phosphate and 3'-hydroxyl ends of adjacent oligonucleotide chains that hybridize to the same complementary target DNA strand. This reaction occurs only when both oligonucleotide chains are fully complementary to the target DNA and there are no gaps between them. Therefore, it can be used for single-base substitution detection, but not the substitute of T4 DNA ligase. Taq DNA Ligase is active at the temperature from 45 to 65°C and requires NAD+ as a coenzyme.","brand":"Cleavion","offers":[{"title":"1000 U","offer_id":57188438081911,"sku":"CLV-0164","price":68.63,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0164-0-cleavion-product.png?v=1781696377"},{"product_id":"exonuclease-iii","title":"Exonuclease III","description":"Exonuclease III is a nuclease that stepwise removes mononucleotides from the 3'-OH termini of double-stranded DNA (dsDNA), and inactive on single-stranded DNA (ssDNA) . The preferred substrates of Exonuclease III are blunt or recessed 3'-termini, although the enzyme also acts at nick sites in double-stranded DNA to generate single-stranded gaps. 3'-overhanging termini are resistant to cleavage; the degree of resistance depends on the length of the extension, with extensions 4 bases or longer being essentially resistant to cleavage. This characteristic can be used to produce strand-specific ssDNA with designed linearized dsDNA having one non-cleavage end (3'-overhang) and the other end designed as a cleavable end (blunt or 5'-overhang), allowing Exonuclease III to digest only one strand. Exonuclease III also exhibits RNase H, 3'-phosphatase, and apurinic\/apyrimidinic-endonuclease activities.","brand":"Cleavion","offers":[{"title":"5000 U","offer_id":57188439884151,"sku":"CLV-0166","price":69.32,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0166-0-cleavion-product.png?v=1781696380"},{"product_id":"t4-polynucleotide-kinase","title":"T4 Polynucleotide Kinase","description":"T4 Polynucleotide Kinase (T4 PNK) is a polyribonucleotide 5'-hydroxyl kinase that catalyzes the transfer of the γ-phosphate group from ATP to the 5'-hydroxyl terminus of oligonucleotide chains, whether they are double-stranded or single-stranded DNA or RNA. Notably, this reaction is reversible. In addition, T4 PNK exhibits 3'-phosphatase activity, which hydrolyzes the 3'-phosphate group from the 3'-phosphate terminus of oligonucleotides, deoxy-3'-monophosphate nucleotides, and deoxy-3'-diphosphate nucleotides.","brand":"Cleavion","offers":[{"title":"500 U","offer_id":57188440506743,"sku":"CLV-0190","price":76.29,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0190-0-cleavion-product.png?v=1781696383"},{"product_id":"hifi-taq-dna-ligase","title":"HiFi Taq DNA Ligase","description":"HiFi Taq DNA Ligase is a mutant type of Taq DNA ligase, which has higher ligation efficiency and high fidelity compared with the wild type. Meanwhile, it maintains the basic functions of the wild type and is a high-temperature-resistant ligase with activity in the range of 37~75°C .Taq DNA ligase, with NAD+ as the coenzyme factor, catalyzes the formation of a phosphodiester bond between the 5'-phosphate and 3'-hydroxyl groups of two adjacent oligonucleotide chains hybridized to the same complementary target DNA strand, and it has significant activity only for DNA nicks. This product is applicable to molecular diagnostic reactions that rely on nick junction, such as ligase detection reaction (LDR), multiplex ligation-dependent probe amplification (MLPA), etc.","brand":"Cleavion","offers":[{"title":"50 Reactions","offer_id":57188441325943,"sku":"CLV-0175","price":114.29,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0175-0-cleavion-product.png?v=1781696387"},{"product_id":"t4-rna-ligase-1","title":"T4 RNA Ligase 1","description":"T4 RNA Ligase 1 is an ATP-dependent ligase derived from bacteriophage T4. It catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3 hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond with hydrolysis of ATP to AMP and PPi. The ligation efficiency of T4 RNA Ligase 1 varies significantly for different substrates, following the order: ssRNA-ssRNA\u0026gt;ssRNA-ssDNA\u0026gt;ssDNA-ssDNA. Additionally, T4 RNA Ligase 1 can also be used for ligation between RNA and mononucleotides, provided that the mononucleotides are phosphorylated at both the 5' and 3' ends. This application is commonly used for 3'-end labeling of RNA.","brand":"Cleavion","offers":[{"title":"1000 U","offer_id":57188441981303,"sku":"CLV-0197","price":96.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0197-0-cleavion-product.png?v=1781696389"},{"product_id":"t4-rna-ligase-2","title":"T4 RNA Ligase 1","description":"T4 RNA Ligase 1 is an ATP-dependent ligase derived from bacteriophage T4. It catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3 hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond with hydrolysis of ATP to AMP and PPi. The ligation efficiency of T4 RNA Ligase 1 varies significantly for different substrates, following the order: ssRNA-ssRNA\u0026gt;ssRNA-ssDNA\u0026gt;ssDNA-ssDNA. Additionally, T4 RNA Ligase 1 can also be used for ligation between RNA and mononucleotides, provided that the mononucleotides are phosphorylated at both the 5' and 3' ends. This application is commonly used for 3'-end labeling of RNA.","brand":"Cleavion","offers":[{"title":"5000 U","offer_id":57188442145143,"sku":"CLV-0198","price":266.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0198-0-cleavion-product.png?v=1781696389"},{"product_id":"assembly-kit-bsmbi-t4-dna-ligase","title":"Assembly Kit (BsmBI+T4 DNA ligase)","description":"Golden Gate Assembly Kit share the same single-step precision cloning strategy, popularly known as \"Golden Gate Assembly\", that relies on the unique properties of type IIs restriction enzymes to generate compatible ends on DNA fragments that are then joined together by the T4 DNA ligase. The Kit are particularly adept at assembling difficult to-clone sequences such as repetitive and very small sequences (\u0026lt;100 bp), gene variants, and TAL (transcription activator-like) effector genes. Type IIS restriction enzymes bind to their recognition sites but cut the DNA downstream from that site at a positional, not sequence specific, cut site. Thus, a single Type IIS restriction enzyme can be used to generate DNA fragments with unique overhangs. The cloning procedure is as follows: Design recognition sites for type IIS restriction enzymes outside the cleavage sites of the target gene. Assembly of digested fragments proceeds through annealing of complementary four base overhangs on adjacent fragments. The digested fragments and the final assembly no longer contain Type IIS restriction enzyme recognition sites, so no further cutting is possible. The vector contains sticky ends complementary to those of the target gene, enabling ligation without introducing extraneous sequences, thereby achieving seamless cloning. The Golden Gate Assembly Kit (BsmBI), based on the above principle, contains all enzymes required for restriction digestion and ligation in a ready-to-use Mix for convenient pipetting. It supports ligation of up to 16 fragments in a single reaction, fully satisfying diverse experimental requirements.","brand":"Cleavion","offers":[{"title":"10 Reactions","offer_id":57188442243447,"sku":"CLV-0199","price":77.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0199-0-cleavion-product.png?v=1781696390"},{"product_id":"assembly-kit-bsmbi-t4-dna-ligase-1","title":"Assembly Kit (BsmBI+T4 DNA ligase)","description":"Golden Gate Assembly Kit share the same single-step precision cloning strategy, popularly known as \"Golden Gate Assembly\", that relies on the unique properties of type IIs restriction enzymes to generate compatible ends on DNA fragments that are then joined together by the T4 DNA ligase. The Kit are particularly adept at assembling difficult to-clone sequences such as repetitive and very small sequences (\u0026lt;100 bp), gene variants, and TAL (transcription activator-like) effector genes. Type IIS restriction enzymes bind to their recognition sites but cut the DNA downstream from that site at a positional, not sequence specific, cut site. Thus, a single Type IIS restriction enzyme can be used to generate DNA fragments with unique overhangs. The cloning procedure is as follows: Design recognition sites for type IIS restriction enzymes outside the cleavage sites of the target gene. Assembly of digested fragments proceeds through annealing of complementary four base overhangs on adjacent fragments. The digested fragments and the final assembly no longer contain Type IIS restriction enzyme recognition sites, so no further cutting is possible. The vector contains sticky ends complementary to those of the target gene, enabling ligation without introducing extraneous sequences, thereby achieving seamless cloning. The Golden Gate Assembly Kit (BsmBI), based on the above principle, contains all enzymes required for restriction digestion and ligation in a ready-to-use Mix for convenient pipetting. It supports ligation of up to 16 fragments in a single reaction, fully satisfying diverse experimental requirements.","brand":"Cleavion","offers":[{"title":"50 Reactions","offer_id":57188442341751,"sku":"CLV-0200","price":132.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0200-0-cleavion-product.png?v=1781696391"}],"url":"https:\/\/cleavion.com\/collections\/ligases-modifying-enzymes.oembed","provider":"Cleavion","version":"1.0","type":"link"}