{"title":"Nucleases, RNases \u0026 DNases","description":"\u003cp\u003eCleavion Nucleases, RNases \u0026amp; DNases includes enzymes for nucleic acid degradation, template removal, RNA handling, DNA cleanup and sample preparation. This category is relevant for molecular biology workflows where controlled digestion or removal of nucleic acids is required.\u003c\/p\u003e\u003cp\u003eTo improve browsing, separate filters should cover enzyme family, substrate preference, grade, unit size and application. ELISA kits for enzyme residual testing should remain in a separate assay-kit collection rather than appearing here as active enzyme reagents.\u003c\/p\u003e","products":[{"product_id":"m-mlv-rnase-h-reverse-transcriptase","title":"M-MLV RNase H-Reverse Transcriptase","description":"M-MLV RNase H- Reverse Transcriptase is a genetically modified and recombinant RNase H-deficient Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase. Through genetic engineering modification, the optimal reaction temperature of this enzyme has been increased to 42 °C , and its extension ability is stronger, making it suitable for longer cDNA synthesis.","brand":"Cleavion","offers":[{"title":"10000 U","offer_id":57188435820919,"sku":"CLV-0033","price":69.71,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0033-0-cleavion-product.png?v=1781696342"},{"product_id":"all-in-one-first-strand-synthesis-master-mix-with-dsdnase","title":"All-in-One First-Strand Synthesis Master Mix (with dsDNase)","description":"The All-in-One First-Strand Synthesis MasterMix (with dsDNase) is a comprehensive kit for first-strand cDNA synthesis, containing reverse transcriptase, reaction buffer, RNAse inhibitor, dNTPs, Oligo (dT) 20VN, and random primers. It provides all the necessary components for efficient cDNA synthesis and requires only the addition of RNA template and water to initiate the reaction. Within just 15 minutes, cDNA fragments up to 12 kb in length can be obtained, which can be utilized downstream in experiments such as qPCR and conventional PCR. This kit incorporates dsDNase, which effectively removes genomic DNA contamination. Unlike conventional DNase I, dsDNase specifically digests double-stranded DNA (dsDNA) and hybrid DNA-RNA molecules while exhibiting thermal sensitivity, enabling rapid and irreversible inactivation under high-temperature conditions. Compared to traditional methods that employ DNase I to remove genomic DNA contamination, the use of dsDNase eliminates the need for additional EDTA for inactivation. This not only saves experimental time but also reduces inhibition of the reverse transcription reaction.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188436115831,"sku":"CLV-0035","price":83.35,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0035-0-cleavion-product.png?v=1781696343"},{"product_id":"rtase-iii-primer-flexible-all-in-one-mix-with-dsdnase","title":"RTase III Primer Flexible All-in-One Mix (with dsDNase)","description":"RTase III Primer Flexible All-in-One Mix is a high-quality, efficient, and convenient one-step cDNA synthesis premix. It is designed to minimize contamination and contains all the necessary components for first-strand cDNA synthesis, including M-MLV GIII Reverse Transcriptase and its reaction buffer, RNase inhibitor, dNTPs, and random primers-all the necessary components. Additionally, it requires the addition of RNA templates, primers, and water to initiate the reaction. This kit can be used with different types of reverse transcription primers to meet diverse experimental needs. Depending on the experimental design, Oligo (dT) 20VN, Random hexamers, or Gene Specific Primers can be selected. This reverse transcription premix allows for the generation of cDNA up to 12 kb in size within 15 minutes. RNA extracted from cells often contains genomic DNA contamination. If the genomic DNA is not removed before reverse transcription, both the genomic DNA and cDNA will be amplified during downstream qPCR reactions (especially when the primers are designed on the same exon), thus affecting the accuracy of gene expression quantification. This kit utilizes dsDNase to efficiently remove genomic DNA contamination. dsDNase can specifically digest double-stranded DNA (dsDNA or the DNA strand in DNA-RNA hybrid chains) and is thermally sensitive, rapidly and irreversibly inactivated at the reverse transcription temperature. Compared to the traditional method of using DNase I to remove genomic DNA contamination, dsDNase does not require the addition of EDTA for inactivation, saving experimental time and reducing inhibition of the reverse transcription reaction.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188436476279,"sku":"CLV-0038","price":93.13,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0038-0-cleavion-product.png?v=1781696346"},{"product_id":"rnase-a","title":"RNase A","description":"Ribonuclease A (RNase A) is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate. RNase A exhibits the highest activity in cleaving single-stranded RNA and demonstrates varying activities under different reaction conditions. At low salt concentrations (0~100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well the RNA strand in RNA-DNA hybrids. However, at NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves single-stranded RNA.","brand":"Cleavion","offers":[{"title":"100 mg","offer_id":57188437393783,"sku":"CLV-0181","price":82.86,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0181-0-cleavion-product.png?v=1781696376"},{"product_id":"rnase-a-1","title":"RNase A","description":"Ribonuclease A (RNase A) is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate. RNase A exhibits the highest activity in cleaving single-stranded RNA and demonstrates varying activities under different reaction conditions. At low salt concentrations (0~100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well the RNA strand in RNA-DNA hybrids. However, at NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves single-stranded RNA.","brand":"Cleavion","offers":[{"title":"1 g","offer_id":57188437590391,"sku":"CLV-0182","price":242.86,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0182-0-cleavion-product.png?v=1781696377"},{"product_id":"rnase-t1","title":"RNase T1","description":"RNase T1 is an endoribonuclease obtained from Aspergillus oryzae, which exhibits specificity for cleaving single-stranded RNA following guanosine ribonucleotides (G), resulting in the formation of 3'-phosphate termini. RNase T1 is capable of forming a nucleoside 2', 3'-cyclic phosphate intermediate to cleave the phosphodiester bond between the 3'-guanosine residue and the 5'-OH group of the adjacent nucleoside, producing oligonucleotides with terminal 3'-GMP and 3'-GMP. The activity of RNase T1 does not depend on metal ions.","brand":"Cleavion","offers":[{"title":"100 KU","offer_id":57188437786999,"sku":"CLV-0183","price":91.07,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0183-0-cleavion-product.png?v=1781696377"},{"product_id":"rnase-t1-1","title":"RNase T1","description":"RNase T1 is an endoribonuclease obtained from Aspergillus oryzae, which exhibits specificity for cleaving single-stranded RNA following guanosine ribonucleotides (G), resulting in the formation of 3'-phosphate termini. RNase T1 is capable of forming a nucleoside 2', 3'-cyclic phosphate intermediate to cleave the phosphodiester bond between the 3'-guanosine residue and the 5'-OH group of the adjacent nucleoside, producing oligonucleotides with terminal 3'-GMP and 3'-GMP. The activity of RNase T1 does not depend on metal ions.","brand":"Cleavion","offers":[{"title":"5x 100 KU","offer_id":57188438049143,"sku":"CLV-0184","price":242.86,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0184-0-cleavion-product.png?v=1781696377"},{"product_id":"thermostable-rnase-t1","title":"Thermostable RNase T1","description":"Thermostable RNase T1 is a thermostable mutant derived from RNase T1 through directed protein evolution. Compared to the wild-type RNase T1, Thermostable RNase T1 retains 100% of its activity at 50°C, while the activity of the wild-type decreases by at least 50% at the same temperature. The higher reaction temperature facilitates the complete unfolding of RNA secondary structures, reducing or avoiding their interference with the enzymatic cleavage process, thereby making the digestion more thorough.","brand":"Cleavion","offers":[{"title":"100 KU","offer_id":57188438245751,"sku":"CLV-0185","price":91.07,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0185-0-cleavion-product.png?v=1781696379"},{"product_id":"dsdnase","title":"dsDNase","description":"DsDNase is a deoxyribonuclease that hydrolyzes phosphodiester bonds in dsDNA, generating oligonucleotides with 5'-phosphate and 3'-hydroxyl ends. dsDNase specifically hydrolyzes dsDNA or the DNA strand in DNA-RNA hybrid chains, while having no effect on RNA and single-stranded DNA. dsDNase is heat-labile and can be rapidly inactivated at 55 °C with DTT. It is primarily used to eliminate genomic DNA contamination in RNA samples before reverse transcription. Compared to the traditional method of using DNase I to eliminate genomic DNA contamination, dsDNase does not require additional EDTA, reduces RNA damage, saves experimental time, and ensures accurate quantification of RNA. The activity of dsDNase can be inhibited by EDTA, SDS, DTT, β-mercaptoethanol and high salt concentrations.","brand":"Cleavion","offers":[{"title":"50 Reactions","offer_id":57188438278519,"sku":"CLV-0165","price":69.32,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0165-0-cleavion-product.png?v=1781696379"},{"product_id":"thermostable-rnase-t1-1","title":"Thermostable RNase T1","description":"Thermostable RNase T1 is a thermostable mutant derived from RNase T1 through directed protein evolution. Compared to the wild-type RNase T1, Thermostable RNase T1 retains 100% of its activity at 50°C, while the activity of the wild-type decreases by at least 50% at the same temperature. The higher reaction temperature facilitates the complete unfolding of RNA secondary structures, reducing or avoiding their interference with the enzymatic cleavage process, thereby making the digestion more thorough.","brand":"Cleavion","offers":[{"title":"5x 100 KU","offer_id":57188439785847,"sku":"CLV-0186","price":242.86,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0186-0-cleavion-product.png?v=1781696380"},{"product_id":"exonuclease-iii","title":"Exonuclease III","description":"Exonuclease III is a nuclease that stepwise removes mononucleotides from the 3'-OH termini of double-stranded DNA (dsDNA), and inactive on single-stranded DNA (ssDNA) . The preferred substrates of Exonuclease III are blunt or recessed 3'-termini, although the enzyme also acts at nick sites in double-stranded DNA to generate single-stranded gaps. 3'-overhanging termini are resistant to cleavage; the degree of resistance depends on the length of the extension, with extensions 4 bases or longer being essentially resistant to cleavage. This characteristic can be used to produce strand-specific ssDNA with designed linearized dsDNA having one non-cleavage end (3'-overhang) and the other end designed as a cleavable end (blunt or 5'-overhang), allowing Exonuclease III to digest only one strand. Exonuclease III also exhibits RNase H, 3'-phosphatase, and apurinic\/apyrimidinic-endonuclease activities.","brand":"Cleavion","offers":[{"title":"5000 U","offer_id":57188439884151,"sku":"CLV-0166","price":69.32,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0166-0-cleavion-product.png?v=1781696380"},{"product_id":"thermostable-rnase-hii","title":"Thermostable RNase HII","description":"This product is derived from the extremely thermophilic bacterium Pyrococcus abyssi (P.abyssi) and obtained through recombinant expression and purification. Thermostable RNase H is an endonuclease that specifically recognizes and cleaves the RNA strand in RNA\/DNA heteroduplexes, without cleaving sSDNA, dsDNA, and exhibits minimal cleavage activity towards single-stranded RNA. Thermostable RNase Hll cleaves at the 5' end of ribonucleotide residues, generating a 5'-phosphate group and a 3'-hydroxyl group after cleavage. This Thermostable RNase Hll exhibits optimal activity within the temperature ranging from 60 to 80°C, remains active between 37°C and 95°C, and exhibits low activity at room temperature. The product is extremely thermostable, with almost no activity loss following incubation at 95°C for 15 minutes, making it compatible with various PCR reaction systems.","brand":"Cleavion","offers":[{"title":"200 U","offer_id":57188440015223,"sku":"CLV-0187","price":114.29,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0187-0-cleavion-product.png?v=1781696381"},{"product_id":"rnase-h","title":"RNase H","description":"RNase H is a ribonuclease endonuclease that specifically cleaves the RNA strand in RNA-DNA hybrids. Its optimal pH is around 8.0. The activity of RNase H is dependent on the presence of Mg2+ or Mn2+.","brand":"Cleavion","offers":[{"title":"1000 U","offer_id":57188440080759,"sku":"CLV-0167","price":63.8,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0167-0-cleavion-product.png?v=1781696382"},{"product_id":"exonuclease-i","title":"Exonuclease I","description":"Exonuclease I (E. coli) is a single-strand specific exonuclease that degrades single-stranded DNA in the 3'-5' direction, releasing 5'-deoxyribonucleotides. Exo I exhibits strong specificity for single-stranded DNA and does not degrade double-stranded DNA or RNA. Additionally, it cannot degrade single-stranded DNA with 3'-OH terminiblocked by phosphoryl or acetyl groups. This product is obtained by recombinant expression of the Exo I gene (E. coli) in Escherichia coli, followed by multiple purification steps.","brand":"Cleavion","offers":[{"title":"1500 U","offer_id":57188440179063,"sku":"CLV-0188","price":72.18,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0188-0-cleavion-product.png?v=1781696381"},{"product_id":"dnase-i-rnase-free","title":"DNase I, RNase-Free","description":"DNase I, RNase-free is an endodeoxyribonuclease that catalyzes to the same extent the degradation of both single- and double-stranded DNA randomly, and produces 5'-P terminal oligonucleotides. The activity of DNase I is dependent on Ca 2+ , and can be activated by bivalent metal ions Mg 2+ , Mn 2+ , etc. In the presence of Mg 2+ , the enzyme can randomly recognize and cut any site on any strand of double-stranded DNA. In the presence of Mn 2+ , nearly identical sites on the two strands of DNA can be recognized and cut, resulting in flat ends or sticky end DNA fragments with 1 to 2 nucleotides overhang.","brand":"Cleavion","offers":[{"title":"1000 U","offer_id":57188440244599,"sku":"CLV-0168","price":93.13,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0168-0-cleavion-product.png?v=1781696382"},{"product_id":"exonuclease-i-1","title":"Exonuclease I","description":"Exonuclease I (E. coli) is a single-strand specific exonuclease that degrades single-stranded DNA in the 3'-5' direction, releasing 5'-deoxyribonucleotides. Exo I exhibits strong specificity for single-stranded DNA and does not degrade double-stranded DNA or RNA. Additionally, it cannot degrade single-stranded DNA with 3'-OH terminiblocked by phosphoryl or acetyl groups. This product is obtained by recombinant expression of the Exo I gene (E. coli) in Escherichia coli, followed by multiple purification steps.","brand":"Cleavion","offers":[{"title":"7500 U","offer_id":57188440375671,"sku":"CLV-0189","price":146.43,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0189-0-cleavion-product.png?v=1781696382"},{"product_id":"omni-nuclease-benzonase","title":"Omni-nuclease (Benzonase)","description":"The Omni-nuclease is the nuclease from Serratia marcescens , also known as Benzonase, obtained through yeast recombinant expression. This enzyme is composed of two subunits, each with a size of 26 kDa. Its activity is 34 times stronger than that of DNase I, efficiently hydrolyzing various forms of DNA and RNA to 5'-monophosphate oligonucleotides with lengths of 3 to 5 bases. The enzyme's activity requires Mg2+, with an optimal pH range of 6 to 10 and an ideal reaction temperature of 37°C . This product is free from bacterial endotoxin residues and maintains high stability and activity under a wide range of conditions, such as 6 M urea, 0.1 M Guanidine HCl, 0.4% Triton X100, 0.1% SDS, 1 mM EDTA, 1 mM PMSF, or 0.4% Sodium deoxycholate. It is highly suitable for the vaccine, protein, and polysaccharide pharmaceutical industries, removing nucleic acid residues from samples or products, thereby enhancing sample purity and the biological efficacy of the products.","brand":"Cleavion","offers":[{"title":"25 KU","offer_id":57188440703351,"sku":"CLV-0171","price":84.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0171-0-cleavion-product.png?v=1781696384"},{"product_id":"omni-nuclease-benzonase-1","title":"Omni-nuclease (Benzonase)","description":"","brand":"Cleavion","offers":[{"title":"50 KU","offer_id":57188440867191,"sku":"CLV-0172","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0172-0-cleavion-product.png?v=1781696384"},{"product_id":"omni-nuclease-benzonase-2","title":"Omni-nuclease (Benzonase)","description":"The Omni-nuclease is the nuclease from Serratia marcescens , also known as Benzonase, obtained through yeast recombinant expression. This enzyme is composed of two subunits, each with a size of 26 kDa. Its activity is 34 times stronger than that of DNase I, efficiently hydrolyzing various forms of DNA and RNA to 5'-monophosphate oligonucleotides with lengths of 3 to 5 bases. The enzyme's activity requires Mg2+, with an optimal pH range of 6 to 10 and an ideal reaction temperature of 37°C . This product is free from bacterial endotoxin residues and maintains high stability and activity under a wide range of conditions, such as 6 M urea, 0.1 M Guanidine HCl, 0.4% Triton X100, 0.1% SDS, 1 mM EDTA, 1 mM PMSF, or 0.4% Sodium deoxycholate. It is highly suitable for the vaccine, protein, and polysaccharide pharmaceutical industries, removing nucleic acid residues from samples or products, thereby enhancing sample purity and the biological efficacy of the products.","brand":"Cleavion","offers":[{"title":"100 KU","offer_id":57188441031031,"sku":"CLV-0173","price":186.35,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0173-0-cleavion-product.png?v=1781696385"},{"product_id":"thermostable-rnase-h","title":"Thermostable RNase H","description":"Thermostable RNase H, is an endoribonuclease that remains active at higher temperatures (above 65 °C) . Thermostable RNase H can selectively identify and cleave the RNA strand in the RNA:DNA heteroduplex, while the DNA in the heteroduplex remains intact. Thermostable RNase H does not degrade single-stranded or double-stranded RNA or DNA.","brand":"Cleavion","offers":[{"title":"200 U","offer_id":57188441194871,"sku":"CLV-0174","price":66.67,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0174-0-cleavion-product.png?v=1781696386"},{"product_id":"dnase-i-rnase-free-lyophilized","title":"DNase I, RNase-Free (Lyophilized)","description":"DNase I, RNase-free is an endodeoxyribonuclease that catalyzes to the same extent the degradation of both single- and double-stranded DNA randomly, and produces 5'-P terminal oligonucleotides. The activity of DNase I is dependent on Ca2+, and can be activated by bivalent metal ions Mg2+, Mn2+, etc. In the presence of Mg2+, the enzyme can randomly recognize and cut any site on any strand of double-stranded DNA. In the presence of Mn2+, nearly identical sites on the two strands of DNA can be recognized and cut, resulting in flat ends or sticky end DNA fragments with 1 to 2 nucleotides overhang. This product is provided in the form of lyophilized powder, which facilitates transportation and storage. However, once dissolved into a solution, please stored it at -20°C for long-term stability.","brand":"Cleavion","offers":[{"title":"1000 U","offer_id":57188441850231,"sku":"CLV-0177","price":95.18,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0177-0-cleavion-product.png?v=1781696388"},{"product_id":"t5-exonuclease","title":"T5 Exonuclease","description":"T5 Exonuclease is a nucleic acid exonuclease that digests DNA from the 5' end in the 5'→3' direction for both double-stranded and single-stranded DNA. It is capable of digesting DNA at nicks or gaps in linear or circular double-stranded DNA, however, it cannot digest supercoiled DNA. In addition, when the Mg2+ concentration in the solution is below 1 mM, the activity of T5 Exonuclease towards single-stranded DNA is inhibited. Consequently, T5 Exonuclease is particularly suitable for degrading linearized and nicked plasmids, removing non-circular DNA from ligation products, seamless cloning (Gibson Assembly), and other applications.","brand":"Cleavion","offers":[{"title":"1000 U","offer_id":57188442014071,"sku":"CLV-0178","price":89.43,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0178-0-cleavion-product.png?v=1781696389"},{"product_id":"t7-endonuclease-i","title":"T7 Endonuclease I","description":"T7 Endonuclease I (T7 Endo I, T7EI), can recognize and cleave imperfectly matched DNA, cruciform DNA structures, Holliday junctions , DNA branch points, and heteroduplex DNA. The cleavage sites are located at the first, second, or third phosphodiester bonds on the 5' side of the mismatch site. Additionally, T7EI can cleave nicked double-stranded DNA, albeit with reduced efficiency. It is noteworthy that T7EI can recognize DNA mismatches resulting from insertions, deletions, or mutations of two or more base pairs (bp) in length, but cannot recognize 1 bp insertions, deletions, or mutations. Furthermore, T7 Endonuclease I does not recognize all types of DNA mismatches and works best with C mismatches. This product is a high-purity protein obtained through cloning and recombinant expression of the T7 Endonuclease I gene in Escherichia coli, followed by purification. It is free of contamination from other endonucleases or exonucleases. (This product is a high-purity protein expressed and purified from E. coli after cloning and expressing the T7 Endonuclease I gene, free from contamination by other endonucleases or exonucleases.)","brand":"Cleavion","offers":[{"title":"250 U","offer_id":57188442177911,"sku":"CLV-0179","price":61.83,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0179-0-cleavion-product.png?v=1781696390"},{"product_id":"t7-endonuclease-i-1","title":"T7 Endonuclease I","description":"T7 Endonuclease I (T7 Endo I, T7EI), can recognize and cleave imperfectly matched DNA, cruciform DNA structures, Holliday junctions , DNA branch points, and heteroduplex DNA. The cleavage sites are located at the first, second, or third phosphodiester bonds on the 5' side of the mismatch site. Additionally, T7EI can cleave nicked double-stranded DNA, albeit with reduced efficiency. It is noteworthy that T7EI can recognize DNA mismatches resulting from insertions, deletions, or mutations of two or more base pairs (bp) in length, but cannot recognize 1 bp insertions, deletions, or mutations. Furthermore, T7 Endonuclease I does not recognize all types of DNA mismatches and works best with C mismatches. This product is a high-purity protein obtained through cloning and recombinant expression of the T7 Endonuclease I gene in Escherichia coli, followed by purification. It is free of contamination from other endonucleases or exonucleases. (This product is a high-purity protein expressed and purified from E. coli after cloning and expressing the T7 Endonuclease I gene, free from contamination by other endonucleases or exonucleases.)","brand":"Cleavion","offers":[{"title":"1250 U","offer_id":57188442308983,"sku":"CLV-0180","price":80.89,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0180-0-cleavion-product.png?v=1781696390"},{"product_id":"aapcas12b-crispr-nuclease","title":"AapCas12b CRISPR Nuclease","description":"AapCas12b is a Type V Cas nuclease, exhibiting good activity across 37~60°C. Guided by crRNA and tracrRNA (or sgRNA), it exhibits cis-cleavage activity against both double-stranded and single-stranded DNA targets. For double-stranded DNA targets, AapCas12b recognizes the site complementary to the crRNA (or sgRNA) spacer sequence downstream of the PAM sequence (5'-TTN) in target DNA, specifically cleaves the target double-stranded DNA to generate sticky ends. For single-stranded DNA targets, its specific cleavage is PAM-independent. After AapCas12b, target DNA and sgRNA form a ternary complex, the enzyme exhibits trans-cleavage activity, namely non-specific cleavage of single-stranded DNA with any sequence. AapCas12b exhibits good activity in common isothermal amplification buffers, making it suitable for rapid nucleic acid detection.","brand":"Cleavion","offers":[{"title":"100 pmol","offer_id":57188442734967,"sku":"CLV-0282","price":137.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0282-0-cleavion-product.png?v=1781696415"},{"product_id":"aapcas12b-crispr-nuclease-1","title":"AapCas12b CRISPR Nuclease","description":"AapCas12b is a Type V Cas nuclease, exhibiting good activity across 37~60°C. Guided by crRNA and tracrRNA (or sgRNA), it exhibits cis-cleavage activity against both double-stranded and single-stranded DNA targets. For double-stranded DNA targets, AapCas12b recognizes the site complementary to the crRNA (or sgRNA) spacer sequence downstream of the PAM sequence (5'-TTN) in target DNA, specifically cleaves the target double-stranded DNA to generate sticky ends. For single-stranded DNA targets, its specific cleavage is PAM-independent. After AapCas12b, target DNA and sgRNA form a ternary complex, the enzyme exhibits trans-cleavage activity, namely non-specific cleavage of single-stranded DNA with any sequence. AapCas12b exhibits good activity in common isothermal amplification buffers, making it suitable for rapid nucleic acid detection.","brand":"Cleavion","offers":[{"title":"1000 pmol","offer_id":57188442898807,"sku":"CLV-0283","price":617.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0283-0-cleavion-product.png?v=1781696415"},{"product_id":"rnase-inhibitor-murine","title":"RNase Inhibitor, Murine","description":"RNase Inhibitor, Murine is a recombinant mouse-derived RNase inhibitor expressed using an Escherichia coli expression system. 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It can fully digest DNA in 300 mM salt. This product is free from RNase contamination.","brand":"Cleavion","offers":[{"title":"1000 U","offer_id":57188443357559,"sku":"CLV-0266","price":103.39,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0266-0-cleavion-product.png?v=1781696418"},{"product_id":"giv-all-in-one-master-mix-with-dsdnase","title":"GIV All-in-One Master Mix with dsDNase","description":"GIV All-in-One MasterMix (with dsDNase) is a simplified system specially designed for single-stranded cDNA synthesis. It contains all components required for the reaction, requiring only the addition of RNA template and water. The M-MLV GIV Reverse Transcriptase in the premix is a newly developed 4th-generation reverse transcriptase. It features faster reaction speed, completing the reverse transcription process within 5 minutes; stronger thermostability with an optimal reaction temperature of 55 °C; the cDNA product can reach up to 20 kb in length. Additionally, it exhibits tolerance to common inhibitors such as ethanol, isopropanol, humic acid, lithium chloride, and guanidine hydrochloride. dsDNase can specifically digest double-stranded DNA even in the presence of primers and probes, without degrading single-stranded DNA or RNA. It exhibits thermal sensitivity, enabling rapid irreversible inactivation at elevated temperatures to eliminate genomic contamination. This prevents dsDNase from damaging DNA within DNA-RNA hybrid strands during reverse transcription.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188445290871,"sku":"CLV-0279","price":360.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0279-0-cleavion-product.png?v=1781696426"},{"product_id":"rnase-gg-for-rna-digestion","title":"RNase GG for RNA Digestion","description":"","brand":"Cleavion","offers":[{"title":"2500 U","offer_id":57188445946231,"sku":"CLV-0303","price":218.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0303-0-cleavion-product.png?v=1781696439"},{"product_id":"i-ceui-homing-endonuclease","title":"I-CeuI Homing Endonuclease","description":"","brand":"Cleavion","offers":[{"title":"250 U","offer_id":57188445978999,"sku":"CLV-0304","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0304-0-cleavion-product.png?v=1781696440"}],"url":"https:\/\/cleavion.com\/collections\/nucleases-rnases-dnases.oembed","provider":"Cleavion","version":"1.0","type":"link"}