{"title":"PCR Enzymes \u0026 DNA Polymerases","description":"\u003cp\u003eCleavion PCR Enzymes \u0026amp; DNA Polymerases brings together core amplification enzymes and hot-start components for routine PCR, high-specificity PCR, strand displacement and molecular biology workflows. This collection includes Taq DNA polymerases, hot-start formats, Taq antibodies, Bst large fragment polymerases, Phi29 DNA polymerase and related amplification enzymes in practical research-use pack sizes.\u003c\/p\u003e\u003cp\u003eUse this category to help researchers quickly select the right polymerase or PCR-support component for standard PCR, isothermal amplification, template extension, multiple displacement amplification or robust DNA synthesis.\u003c\/p\u003e","products":[{"product_id":"taq-dna-polymerase","title":"Taq DNA Polymerase","description":"Taq DNA Polymerase is a thermostable DNA polymerase derived from the thermophilic bacterium Thermus aquaticus. It is obtained through recombinant expression and purification in Escherichia coli, and it has a molecular weight of 94 kDa. This enzyme exhibits 5'→3' polymerase activity and 5'→3' exonuclease activity, but lacks 3'→5' exonuclease activity.","brand":"Cleavion","offers":[{"title":"1000 U","offer_id":57188434018679,"sku":"CLV-0001","price":69.26,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0001-0-cleavion-product.png?v=1781696333"},{"product_id":"2-pcr-master-mix","title":"2× PCR Master Mix","description":"The 2x S705 HiFi Master Mix is a ready-to-use 2x premix that contains S705 High-Fidelity DNA Polymerase, dNTPs, and a optimized reaction buffer for PCR. It only requires the addition of template, primers, and Nuclease-Free Water to perform high-fidelity PCR amplification. S705 High-Fidelity DNA Polymerase is a high-fidelity DNA polymerase obtained through directed evolution.Its fidelity is 70-fold higher than that of Taq polymerase, and its an amplification speed is five times faster than that of Pfu polymerase. The 2x S705 HiFi Master Mix is formulated with unique extension factors and specificity enhancers, which significantly improves the capacity for long fragment amplification, specificity, and product yield. When using simple templates such as λDNA and plasmids, the 2x S705 HiFi Master Mix can easily amplify fragments up to 20 kb in length. When using complex templates like genomic DNA, it can effectively amplify fragments up to 12 kb. Furthermore, the 2x S705 HiFi Master Mix demonstrates good tolerance to PCR inhibitors, enabling excellent amplification even from crude templates.","brand":"Cleavion","offers":[{"title":"1 mL","offer_id":57188433985911,"sku":"CLV-0021","price":63.8,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0021-0-cleavion-product.png?v=1781696333"},{"product_id":"taq-hs-sybr-green-qpcr-premix-universal","title":"Taq-HS SYBR Green qPCR Premix (Universal)","description":"Taq-HS SYBR® Green qPCR Premix is a special premix for qPCR detection using SYBR® Green I chimeric fluorescence method. The Mix contains all components for qPCR, except DNA templates and primers. It reduces the reaction set-up time and risk of contaminations. The core component is the chemically modified hot-start Taq DNA polymerase. With the optimized buffer, the Mix suppresses non-specific amplifications well to obtain consistent and reliable qPCR results in a broad template concentration range. This product contains universial correction dye that are compatible with all of qPCR devices, including instruments that require ROX calibration, and do not require additional dyes to correct the instrument.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188434116983,"sku":"CLV-0041","price":82.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0041-0-cleavion-product.png?v=1781696334"},{"product_id":"2-pcr-master-mix-1","title":"2× PCR Master Mix","description":"The 2x S705 HiFi Master Mix is a ready-to-use 2x premix that contains S705 High-Fidelity DNA Polymerase, dNTPs, and a optimized reaction buffer for PCR. It only requires the addition of template, primers, and Nuclease-Free Water to perform high-fidelity PCR amplification. S705 High-Fidelity DNA Polymerase is a high-fidelity DNA polymerase obtained through directed evolution.Its fidelity is 70-fold higher than that of Taq polymerase, and its an amplification speed is five times faster than that of Pfu polymerase. The 2x S705 HiFi Master Mix is formulated with unique extension factors and specificity enhancers, which significantly improves the capacity for long fragment amplification, specificity, and product yield. When using simple templates such as λDNA and plasmids, the 2x S705 HiFi Master Mix can easily amplify fragments up to 20 kb in length. When using complex templates like genomic DNA, it can effectively amplify fragments up to 12 kb. Furthermore, the 2x S705 HiFi Master Mix demonstrates good tolerance to PCR inhibitors, enabling excellent amplification even from crude templates.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188434149751,"sku":"CLV-0022","price":99.45,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0022-0-cleavion-product.png?v=1781696334"},{"product_id":"taq-hs-dna-polymerase","title":"Taq-HS DNA Polymerase","description":"","brand":"Cleavion","offers":[{"title":"1000 U","offer_id":57188434215287,"sku":"CLV-0002","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0002-0-cleavion-product.png?v=1781696334"},{"product_id":"taq-hs-sybr-green-qpcr-premix-rox-separate","title":"Taq-HS SYBR Green qPCR Premix (ROX Separate)","description":"Taq-HS SYBR® Green qPCR Premix is a special premix for qPCR detection using SYBR® Green I chimeric fluorescence method. The Mix contains all components for qPCR, except DNA templates and primers. It reduces the reaction set-up time and risk of contaminations. The core component is the chemically modified hot-start Taq DNA polymerase. With the optimized buffer, the Mix suppresses non-specific amplifications well to obtain consistent and reliable qPCR results in a broad template concentration range.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188434280823,"sku":"CLV-0042","price":78.91,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0042-0-cleavion-product.png?v=1781696335"},{"product_id":"2-pcr-master-mix-2","title":"2× PCR Master Mix","description":"The 2x S705 HiFi Master Mix is a ready-to-use 2x premix that contains S705 High-Fidelity DNA Polymerase, dNTPs, and a optimized reaction buffer for PCR. It only requires the addition of template, primers, and Nuclease-Free Water to perform high-fidelity PCR amplification. S705 High-Fidelity DNA Polymerase is a high-fidelity DNA polymerase obtained through directed evolution.Its fidelity is 70-fold higher than that of Taq polymerase, and its an amplification speed is five times faster than that of Pfu polymerase. The 2x S705 HiFi Master Mix is formulated with unique extension factors and specificity enhancers, which significantly improves the capacity for long fragment amplification, specificity, and product yield. When using simple templates such as λDNA and plasmids, the 2x S705 HiFi Master Mix can easily amplify fragments up to 20 kb in length. When using complex templates like genomic DNA, it can effectively amplify fragments up to 12 kb. Furthermore, the 2x S705 HiFi Master Mix demonstrates good tolerance to PCR inhibitors, enabling excellent amplification even from crude templates.","brand":"Cleavion","offers":[{"title":"20x 1 mL","offer_id":57188434411895,"sku":"CLV-0023","price":316.14,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0023-0-cleavion-product.png?v=1781696335"},{"product_id":"abtaq-dna-polymerase","title":"AbTaq DNA Polymerase","description":"AbTaq DNA Polymerase is a hot-start Taq DNA Polymerase modified with monoclonal antibodies. The antibodies effectively inhibit the DNA polymerase activity below 55°C, preventing non-specific amplification at lower temperatures. At high temperatures, the antibodies irreversibly dissociate, allowing the polymerase to regain its activity. This hot-start feature ensures specific amplification and helps to avoid non-specific amplification at low temperatures.","brand":"Cleavion","offers":[{"title":"500 U","offer_id":57188434510199,"sku":"CLV-0003","price":162.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0003-0-cleavion-product.png?v=1781696335"},{"product_id":"taq-sybr-green-qpcr-premix-no-rox","title":"Taq SYBR Green qPCR Premix (No ROX)","description":"Taq SYBR® Green qPCR Premix is a special premix for qPCR detection using SYBR Green I chimeric fluorescence method. The Mix contains all components for qPCR, except DNA templates and primers. It reduces the reaction set-up time and risk of contaminations. The core component is the antibody modified hot-start Taq DNA polymerase. With the optimized buffer and PCR enhancer, the Mix has high efficiency and specificity that suppresses non-specific amplifications well to obtain consistent and reliable qPCR results in a broad template concentration range.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188434542967,"sku":"CLV-0043","price":85.49,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0043-0-cleavion-product.png?v=1781696336"},{"product_id":"2-pcr-master-mix-with-dye","title":"2× PCR Master Mix (with Dye)","description":"The 2x S705 HiFi Master Mix (+Dye) is a ready-to-use 2x premix that contains S705 High-Fidelity DNA Polymerase, dNTPs, and a optimized reaction buffer for PCR. It only requires the addition of template, primers, and Nuclease-Free Water to perform high-fidelity PCR amplification. S705 High-Fidelity DNA Polymerase is a high-fidelity DNA polymerase obtained through directed evolution.Its fidelity is 70-fold higher than that of Taq polymerase, and its an amplification speed is five times faster than that of Pfu polymerase. The 2x S705 HiFi Master Mix (+Dye) is formulated with unique extension factors and specificity enhancers, which significantly improves the capacity for long fragment amplification, specificity, and product yield. When using simple templates such as λDNA and plasmids, the 2x S705 HiFi Master Mix (+Dye) can easily amplify fragments up to 20 kb in length. When using complex templates like genomic DNA, it can effectively amplify fragments up to 12 kb. Furthermore, the 2x S705 HiFi Master Mix (+Dye) demonstrates good tolerance to PCR inhibitors, enabling excellent amplification even from crude templates. The premix contains red and yellow tracking dyes and PCR products can be directly loaded for electrophoresis without adding a Loading Buffer. In 1% agarose gel, the red dye has a migration rate comparable to that of the 1500 bp dsDNA fragment and the yellow dye has a migration rate comparable to that of the 10 bp dsDNA fragment.","brand":"Cleavion","offers":[{"title":"1 mL","offer_id":57188434575735,"sku":"CLV-0024","price":63.8,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0024-0-cleavion-product.png?v=1781696336"},{"product_id":"taq-antibody","title":"Taq Antibody","description":"Taq antibody is a mouse monoclonal antibody specific bound to Taq DNA polymerase. It can effectively inhibit over 95% of Taq DNA polymerase activity at 55°C. Heating at 95°C for 30 seconds, the antibody irreversibly dissociates from Taq DNA polymerase, release the activity of polymerase .","brand":"Cleavion","offers":[{"title":"200 U","offer_id":57188434674039,"sku":"CLV-0004","price":60.35,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0004-0-cleavion-product.png?v=1781696336"},{"product_id":"taq-sybr-green-qpcr-premix-universal","title":"Taq SYBR Green qPCR Premix (Universal)","description":"Taq SYBR® Green qPCR Premix (Universal) is a special premix for qPCR detection using SYBR Green I chimeric fluorescence method. The Mix contains all components for qPCR, except DNA templates and primers. It reduces the reaction set-up time and risk of contaminations. The core component is the antibody modified hot-start Taq DNA polymerase. With the optimized buffer and PCR enhancer, the Mix has high efficiency and specificity that suppresses non-specific amplifications well to obtain consistent and reliable qPCR results in a broad template concentration range. This product contains universial correction dye that are compatible with all of qPCR devices, including instruments that require ROX calibration, and do not require additional dyes to correct the instrument.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188434706807,"sku":"CLV-0044","price":66.44,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0044-0-cleavion-product.png?v=1781696338"},{"product_id":"2-pcr-master-mix-with-dye-1","title":"2× PCR Master Mix (with Dye)","description":"The 2x S705 HiFi Master Mix (+Dye) is a ready-to-use 2x premix that contains S705 High-Fidelity DNA Polymerase, dNTPs, and a optimized reaction buffer for PCR. It only requires the addition of template, primers, and Nuclease-Free Water to perform high-fidelity PCR amplification. S705 High-Fidelity DNA Polymerase is a high-fidelity DNA polymerase obtained through directed evolution.Its fidelity is 70-fold higher than that of Taq polymerase, and its an amplification speed is five times faster than that of Pfu polymerase. The 2x S705 HiFi Master Mix (+Dye) is formulated with unique extension factors and specificity enhancers, which significantly improves the capacity for long fragment amplification, specificity, and product yield. When using simple templates such as λDNA and plasmids, the 2x S705 HiFi Master Mix (+Dye) can easily amplify fragments up to 20 kb in length. When using complex templates like genomic DNA, it can effectively amplify fragments up to 12 kb. Furthermore, the 2x S705 HiFi Master Mix (+Dye) demonstrates good tolerance to PCR inhibitors, enabling excellent amplification even from crude templates. The premix contains red and yellow tracking dyes and PCR products can be directly loaded for electrophoresis without adding a Loading Buffer. In 1% agarose gel, the red dye has a migration rate comparable to that of the 1500 bp dsDNA fragment and the yellow dye has a migration rate comparable to that of the 10 bp dsDNA fragment.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188434739575,"sku":"CLV-0025","price":99.45,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0025-0-cleavion-product.png?v=1781696336"},{"product_id":"2-pcr-master-mix-with-dye-2","title":"2× PCR Master Mix (with Dye)","description":"The 2x S705 HiFi Master Mix (+Dye) is a ready-to-use 2x premix that contains S705 High-Fidelity DNA Polymerase, dNTPs, and a optimized reaction buffer for PCR. It only requires the addition of template, primers, and Nuclease-Free Water to perform high-fidelity PCR amplification. S705 High-Fidelity DNA Polymerase is a high-fidelity DNA polymerase obtained through directed evolution.Its fidelity is 70-fold higher than that of Taq polymerase, and its an amplification speed is five times faster than that of Pfu polymerase. The 2x S705 HiFi Master Mix (+Dye) is formulated with unique extension factors and specificity enhancers, which significantly improves the capacity for long fragment amplification, specificity, and product yield. When using simple templates such as λDNA and plasmids, the 2x S705 HiFi Master Mix (+Dye) can easily amplify fragments up to 20 kb in length. When using complex templates like genomic DNA, it can effectively amplify fragments up to 12 kb. Furthermore, the 2x S705 HiFi Master Mix (+Dye) demonstrates good tolerance to PCR inhibitors, enabling excellent amplification even from crude templates. The premix contains red and yellow tracking dyes and PCR products can be directly loaded for electrophoresis without adding a Loading Buffer. In 1% agarose gel, the red dye has a migration rate comparable to that of the 1500 bp dsDNA fragment and the yellow dye has a migration rate comparable to that of the 10 bp dsDNA fragment.","brand":"Cleavion","offers":[{"title":"20x 1 mL","offer_id":57188434870647,"sku":"CLV-0026","price":316.14,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0026-0-cleavion-product.png?v=1781696337"},{"product_id":"taq-sybr-green-qpcr-premix-rox-separate","title":"Taq SYBR Green qPCR Premix (ROX Separate)","description":"Taq SYBR® Green qPCR Premix is a special premix for qPCR detection using SYBR Green I chimeric fluorescence method. The Mix contains all components for qPCR, except DNA templates and primers. It reduces the reaction set-up time and risk of contaminations. The core component is the antibody modified hot-start Taq DNA polymerase. With the optimized buffer and PCR enhancer, the Mix has high efficiency and specificity that suppresses non-specific amplifications well to obtain consistent and reliable qPCR results in a broad template concentration range.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188434903415,"sku":"CLV-0045","price":85.49,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0045-0-cleavion-product.png?v=1781696337"},{"product_id":"phi29-dna-polymerase","title":"phi29 DNA Polymerase","description":"Phi29 DNA Polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29. phi29 DNA Polymerase exhibits a strong strand-displacement activity, a strong chain affinity, and a single polymerization can achieve a continuous polymerization extension up to 70 kb. In addition, phi29 DNA Polymerase has extremely high fidelity due to its inherent 3'→5' exonuclease activity. However, because of the enzymes's 3'→5' proofreading exonuclease, 3'-modified primers are highly recommended.","brand":"Cleavion","offers":[{"title":"500 U","offer_id":57188435001719,"sku":"CLV-0027","price":70.54,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0027-0-cleavion-product.png?v=1781696337"},{"product_id":"sybr-qpcr-mix-universal","title":"SYBR qPCR Mix (Universal)","description":"F488 SYBR qPCR Mix (Universal) is a special premix for qPCR detection using SYBR Green I chimeric fluorescence method. The Mix contains all components for qPCR, except DNA templates and primers. The core component is the updated hot-start Taq DNA polymerase with better specificity. With the optimized buffer, the mix is able to obtain consistent and reliable qPCR results in a broad template concentration range. This product contains universial reference dye compatible with most of qPCR devices, and do not require additional ROX dyes before reaction.","brand":"Cleavion","offers":[{"title":"25x 1 mL","offer_id":57188435100023,"sku":"CLV-0046","price":157.14,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0046-0-cleavion-product.png?v=1781696338"},{"product_id":"phi29-dna-polymerase-1","title":"phi29 DNA Polymerase","description":"Phi29 DNA Polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29. phi29 DNA Polymerase exhibits a strong strand-displacement activity, a strong chain affinity, and a single polymerization can achieve a continuous polymerization extension up to 70 kb. In addition, phi29 DNA Polymerase has extremely high fidelity due to its inherent 3'→5' exonuclease activity. However, because of the enzymes's 3'→5' proofreading exonuclease, 3'-modified primers are highly recommended.","brand":"Cleavion","offers":[{"title":"2500 U","offer_id":57188435165559,"sku":"CLV-0028","price":139.29,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0028-0-cleavion-product.png?v=1781696339"},{"product_id":"taq-hs-probe-qpcr-premix-no-rox","title":"Taq-HS Probe qPCR Premix (No ROX)","description":"Taq-HS Probe qPCR Premix is a 2x pre-mixed solution developed for real-time quantitative PCR based on the unique “double-block”hot-start Taq DNA polymerase. This product contains all the fluorescent quantitative PCR components except for primers and sample DNA, reducing operational steps, shortening the time, and lowering the risk of contamination. lt is ideal for TaqMan probe-based detection using cDNA or DNA as the template. Due to design variations in some brands and models of fluorescent quantitative PCR instruments, there may be slight differences in the fluorescence signals between PCR wells. Therefore, the addition of a reference dye is necessary for calibration. This product is provided with ROX Reference Dye at different concentrations, please select the appropriate one according to the specific instrument model.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188435231095,"sku":"CLV-0047","price":85.49,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0047-0-cleavion-product.png?v=1781696339"},{"product_id":"2-taq-pcr-mix","title":"2× Taq PCR Mix","description":"Taq PCR Aurora Mix is a ready-to-use PCR mix with the concentration of 2x. lt offers convenience and reduces the risk of contamination. PCR products synthesized with this mix have an overhang of A bases at the 3' end, enabling direct T\/A cloning after purification. The Mix contains Taq DNA polymerase and a protein with 3'→5' exonuclease activity, enabling efficient amplification of DNA fragments up to 7 kb with high yields. Coupled with the optimized reaction buffer, it can achieve efficient amplification for different GC contents (ranging from 30% to 70%) . In addition, compared with WT-Taq DNA polymerase, the extension rate of Taq PCR Aurora Mix is enhanced by 2 to 4 times, which can effectively shorten the reaction time. This PCR Mix contains two dyes. The PCR products can be directly loaded for electrophoresis without adding a Loading Buffer, and two indicator bands in blue and red colors will appear during the electrophoresis process. These dyes do not interfere with the efficiency of PCR amplification. However, for experiments that require optical analyses such as absorbance and fluorescence of the PCR products, it is recommended to purify the PCR products prior to analysis.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188435296631,"sku":"CLV-0029","price":59.86,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0029-0-cleavion-product.png?v=1781696339"},{"product_id":"taq-hs-probe-qpcr-premix-universal","title":"Taq-HS Probe qPCR Premix (Universal)","description":"Taq-HS Probe qPCR Premix is a 2x pre-mixed solution developed for real-time quantitative PCR based on the unique “double-block”hot-start Taq DNA polymerase. This product contains all the fluorescent quantitative PCR components except for primers and sample DNA, reducing operational steps, shortening the time, and lowering the risk of contamination. lt is ideal for TaqMan probe-based detection using cDNA or DNA as the template. This product contains universial correction dye that are compatible with all of qPCR devices, including instruments that require ROX calibration, and do not require additional dyes to correct the instrument.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188435427703,"sku":"CLV-0048","price":89.43,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0048-0-cleavion-product.png?v=1781696340"},{"product_id":"2-taq-pcr-mix-1","title":"2× Taq PCR Mix","description":"Taq PCR Aurora Mix is a ready-to-use PCR mix with the concentration of 2x. lt offers convenience and reduces the risk of contamination. PCR products synthesized with this mix have an overhang of A bases at the 3' end, enabling direct T\/A cloning after purification. The Mix contains Taq DNA polymerase and a protein with 3'→5' exonuclease activity, enabling efficient amplification of DNA fragments up to 7 kb with high yields. Coupled with the optimized reaction buffer, it can achieve efficient amplification for different GC contents (ranging from 30% to 70%) . In addition, compared with WT-Taq DNA polymerase, the extension rate of Taq PCR Aurora Mix is enhanced by 2 to 4 times, which can effectively shorten the reaction time. This PCR Mix contains two dyes. The PCR products can be directly loaded for electrophoresis without adding a Loading Buffer, and two indicator bands in blue and red colors will appear during the electrophoresis process. These dyes do not interfere with the efficiency of PCR amplification. However, for experiments that require optical analyses such as absorbance and fluorescence of the PCR products, it is recommended to purify the PCR products prior to analysis.","brand":"Cleavion","offers":[{"title":"100x 1 mL","offer_id":57188435460471,"sku":"CLV-0030","price":194.64,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0030-0-cleavion-product.png?v=1781696340"},{"product_id":"bsu-dna-polymerase-large-fragment","title":"Bsu DNA Polymerase (Large Fragment)","description":"Bsu DNA Polymerase (Large Fragment) is derived from Bacillus subtilis , and the N-terminal 296 amino acids of Bacillus subtilis DNA Polymerase I are removed. The enzyme retains the 5'→3' polymerase activity of Bacillus subtilis DNA Polymerase I but lacks 5'→3' and 3'→5' exonuclease activities. The enzyme has an optimal reaction temperature of 37°C , exhibits strong strand displacement activity, and is suitable for DNA isothermal amplification or cDNA synthesis.","brand":"Cleavion","offers":[{"title":"500 µg","offer_id":57188435526007,"sku":"CLV-0009","price":353.05,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0009-0-cleavion-product.png?v=1781696339"},{"product_id":"taq-plus-pcr-mix-2","title":"Taq-Plus PCR Mix (2×)","description":"Taq-Plus PCR Forest Mix (2x) V2 is a pre-mixed PCR reaction solution formulated with Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. It is capable of robust amplification of up to 6 kb from genomic DNA. PCR products amplified with this mix have a 3'-terminal adenosine (A), and therefore PCR products can be used directly for T\/A cloning. The Forest mix is supplemented with two tracking dyes that allows for direct loading of the PCR product on a gel. The dyes in the mix do not interfere with PCR performance but need to purify the PCR product when it's used for absorbance, fluorescence, etc. In addition, the dye-free Taq-Plus PCR Master Mix (2x) V2 can also be used.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188435558775,"sku":"CLV-0031","price":58.28,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0031-0-cleavion-product.png?v=1781696341"},{"product_id":"one-step-rt-qpcr-probe-kit","title":"One Step RT-qPCR Probe Kit","description":"One Step RT-qPCR Probe Kit v2 is a kit for multiple reverse transcription - fluorescent quantitative PCR reactions using RNA as a template. During the experiment, reverse transcription and quantitative PCR are performed in the same reaction tube, which simplifies the experimental operation and reduces the risk of contamination. The kit contains thermo-stable Reverse Transcriptase to efficiently synthesize the first-strand cDNA, and contain hot-start Ab-Taq DNA Polymerase to quantitative amplification, making it suitable for probe based qPCR. The product already includes all components for reverse transcription - fluorescent quantitative PCR, excluding primers and sample RNA, including RNase inhibitors, RTase, and Ab-Taq. It can reduce operation steps, shorten sample addition time, and lower the risk of contamination.","brand":"Cleavion","offers":[{"title":"250 Reactions","offer_id":57188435591543,"sku":"CLV-0049","price":164.29,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0049-0-cleavion-product.png?v=1781696341"},{"product_id":"taq-plus-pcr-mix-3","title":"Taq-Plus PCR Mix (2×)","description":"Taq-Plus PCR Forest Mix (2x) V2 is a pre-mixed PCR reaction solution formulated with Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. It is capable of robust amplification of up to 6 kb from genomic DNA. PCR products amplified with this mix have a 3'-terminal adenosine (A), and therefore PCR products can be used directly for T\/A cloning. The Forest mix is supplemented with two tracking dyes that allows for direct loading of the PCR product on a gel. The dyes in the mix do not interfere with PCR performance but need to purify the PCR product when it's used for absorbance, fluorescence, etc. In addition, the dye-free Taq-Plus PCR Master Mix (2x) V2 can also be used.","brand":"Cleavion","offers":[{"title":"100x 1 mL","offer_id":57188435657079,"sku":"CLV-0032","price":239.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0032-0-cleavion-product.png?v=1781696341"},{"product_id":"taq-sybr-qpcr-premix-universal","title":"Taq SYBR qPCR Premix (Universal)","description":"Taq Visual SYBR qPCR Premix (Universal) is a qPCR reagent specifically designed for SYBR Green I intercalating dye-based assays. This 2x premix includes all necessary qPCR components except for primers and DNA samples, thereby reducing operational steps, shortening sample loading time, and minimizing the risk of contamination. Its core component is an antibody-modified hot-start Taq DNA polymerase, combined with a meticulously optimized buffer system and PCR reaction enhancers. This formulation provides strong specificity and high amplification efficiency, effectively inhibiting non-specific amplification and enabling accurate quantification of templates across a wide concentration range, resulting in stable and reliable qPCR results. This product already contains a universal reference dye and is compatible with most qPCR instruments, eliminating the need for additional dyes for instrument calibration. This product utilizes the color-changing effect resulting from the mixing of different dyes to track the process, thereby significantly minimizing pipetting errors. Taq Visual SYBR qPCR Premix contains a blue dye, while the 10x Dilution Buffer contains a yellow dye. When Taq Visual SYBR qPCR Premix (blue) is mixed with template diluted in Dilution Buffer (yellow), a color change from blue to green occurs, allowing for an accurate visual confirmation of template addition based on the liquid color","brand":"Cleavion","offers":[{"title":"4x 1.25 mL","offer_id":57188435722615,"sku":"CLV-0050","price":68.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0050-0-cleavion-product.png?v=1781696341"},{"product_id":"taq-sybr-qpcr-premix-universal-1","title":"Taq SYBR qPCR Premix (Universal)","description":"","brand":"Cleavion","offers":[{"title":"20x 1.25 mL","offer_id":57188435886455,"sku":"CLV-0051","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0051-0-cleavion-product.png?v=1781696342"},{"product_id":"all-in-one-first-strand-synthesis-master-mix-with-dsdnase","title":"All-in-One First-Strand Synthesis Master Mix (with dsDNase)","description":"The All-in-One First-Strand Synthesis MasterMix (with dsDNase) is a comprehensive kit for first-strand cDNA synthesis, containing reverse transcriptase, reaction buffer, RNAse inhibitor, dNTPs, Oligo (dT) 20VN, and random primers. It provides all the necessary components for efficient cDNA synthesis and requires only the addition of RNA template and water to initiate the reaction. Within just 15 minutes, cDNA fragments up to 12 kb in length can be obtained, which can be utilized downstream in experiments such as qPCR and conventional PCR. This kit incorporates dsDNase, which effectively removes genomic DNA contamination. Unlike conventional DNase I, dsDNase specifically digests double-stranded DNA (dsDNA) and hybrid DNA-RNA molecules while exhibiting thermal sensitivity, enabling rapid and irreversible inactivation under high-temperature conditions. Compared to traditional methods that employ DNase I to remove genomic DNA contamination, the use of dsDNase eliminates the need for additional EDTA for inactivation. This not only saves experimental time but also reduces inhibition of the reverse transcription reaction.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188436115831,"sku":"CLV-0035","price":83.35,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0035-0-cleavion-product.png?v=1781696343"},{"product_id":"all-in-one-first-strand-synthesis-master-mix","title":"All-in-One First-Strand Synthesis Master Mix","description":"All-in-One First-Strand Synthesis MasterMix is a high-quality, efficient, and convenient one-step cDNA synthesis mix. It is designed to minimize contamination and contains all the necessary components for first-strand cDNA synthesis, including thermostable M-MLV GIII Reverse Transcriptase and its reaction buffer, RNase inhibitor, dNTPs, Oligo (dT) 20VN, and random primers-all the necessary components. Simply add RNA template and water to initiate the reaction. Using the reverse transcription pre-mix, cDNA of up to 12 kb in size can be obtained within 15 minutes.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188436246903,"sku":"CLV-0036","price":86.8,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0036-0-cleavion-product.png?v=1781696344"},{"product_id":"taq-hs-sybr-green-qpcr-premix-no-rox","title":"Taq-HS SYBR Green qPCR Premix (No ROX)","description":"Taq-HS SYBR® Green qPCR Premix is a special premix for qPCR detection using SYBR® Green I chimeric fluorescence method. The Mix contains all components for qPCR, except DNA templates and primers. It reduces the reaction set-up time and risk of contaminations. The core component is the chemically modified hot-start Taq DNA polymerase. With the optimized buffer, the Mix suppresses non-specific amplifications well to obtain consistent and reliable qPCR results in a broad template concentration range.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188436771191,"sku":"CLV-0040","price":78.91,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0040-0-cleavion-product.png?v=1781696347"},{"product_id":"bst-ii-dna-polymerase-large-fragment","title":"Bst II DNA Polymerase (Large Fragment)","description":"Bst II DNA Polymerase (Large Fragment) is a modified form of Bst DNA polymerase obtained by truncating the 5' → 3' exonuclease domain. Through directed evolution and recombinant expression in Escherichia coli , this enzyme has been engineered. It possesses robust 5' → 3' DNA polymerase activity and strand displacement activity. The optimal reaction temperature for this enzyme is 65°C, and it is suitable for various isothermal amplification reactions.Compared to the wild-type enzyme, Bst II DNA Polymerase (Large Fragment) exhibits faster amplification rates, enhanced tolerance to dUTP and salt, as well as improved stability.","brand":"Cleavion","offers":[{"title":"1600 U","offer_id":57188436836727,"sku":"CLV-0017","price":77.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0017-0-cleavion-product.png?v=1781696347"},{"product_id":"bst-ii-dna-polymerase-large-fragment-1","title":"Bst II DNA Polymerase (Large Fragment)","description":"Bst II DNA Polymerase (Large Fragment) is a modified form of Bst DNA polymerase obtained by truncating the 5' → 3' exonuclease domain. Through directed evolution and recombinant expression in Escherichia coli , this enzyme has been engineered. It possesses robust 5' → 3' DNA polymerase activity and strand displacement activity. The optimal reaction temperature for this enzyme is 65°C, and it is suitable for various isothermal amplification reactions.Compared to the wild-type enzyme, Bst II DNA Polymerase (Large Fragment) exhibits faster amplification rates, enhanced tolerance to dUTP and salt, as well as improved stability.","brand":"Cleavion","offers":[{"title":"8000 U","offer_id":57188436935031,"sku":"CLV-0018","price":191.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0018-0-cleavion-product.png?v=1781696348"},{"product_id":"bst-ii-dna-polymerase-large-fragment-glycerol-free","title":"Bst II DNA Polymerase (Large Fragment, Glycerol Free)","description":"Bst II DNA Polymerase (Large Fragment, Glycerol Free) is a modified version of Bacillus stearothermophilus DNA polymerase. It has been engineered and expressed in Escherichia coli. This product exhibits strong 5'→3' DNA polymerase activity and strand displacement activity but lacks 5'→3' exonuclease activity, and the optimal reaction temperature is 65°C. lt is suitable for various isothermal amplification reactions, such as Loop-Mediated lsothermal Amplification (LAMP), Rolling Circle Amplification (RCA), Helicase-Dependent Amplification (HDA), etc. Compared to the wild-type enzyme, Bst II DNAPolymerase (Large Fragment, Glycerol Free) has a faster amplification rate, stronger dUTP tolerance, salt tolerance, and improved stability. This product is glycerol-free and can be used for the preparation of various lyophilized reagents. Avoid repeated freeze-thaw.","brand":"Cleavion","offers":[{"title":"1600 U","offer_id":57188437033335,"sku":"CLV-0019","price":77.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0019-0-cleavion-product.png?v=1781696349"},{"product_id":"taq-antibody-b8","title":"Taq Antibody (B8)","description":"Taq-antibody (B8) is a monoclonal antibody of murine origin that binds Taq polymerase and inhibits its activity efficiently. This antibody is able to bind to Taq DNA Polymerase so strongly that inhibits 95% activity of Polymerase at 55°C. Therefore, it can effectively suppress the degradation of mismatched probes or other materials at low temperatures, reducing non-specific signals. After heated at 95°Cfor 30 seconds, the enzyme-antibody complex dissociates, and the Taq-antibody (B8) becomes nonfunctional. Once the antibody is denatured, the activity of the Taq DNA polymerase is restored and the enzyme functions normally during the PCR reaction. This product is especially suitable for Quantitative Real-Time PCR based on probe method. Exonuclease activity-blocking antibodies and polymerase activity-blocking antibodies can be used in combination to obtain double-blocking antibodies. When these antibodies bind to Taq DNA polymerase, they can simultaneously inhibit both polymerase and exonuclease activities, , further improving the specificity of the reaction.","brand":"Cleavion","offers":[{"title":"100 µL","offer_id":57188437131639,"sku":"CLV-0020","price":75.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0020-0-cleavion-product.png?v=1781696349"},{"product_id":"2x-taq-as-pcr-master-mix-with-loading-dye","title":"2x Taq-AS PCR Master Mix with Loading Dye","description":"The 2xTaq-AS PCR Mix (+Dye) includes Taq-AS DNA Polymerase, reaction buffer, dNTPs, and loading dye among other components. Taq-AS (Advanced Strong) DNA Polymerase is a screened variant capable of efficiently amplifying DNA fragments up to 5 kb. It exhibits excellent inhibitor tolerance and has a threefold faster amplification speed compared to regular Taq DNA polymerase. The PCR products do not require the addition of a loading buffer and can be directly loaded for gel electrophoresis. During the electrophoresis process, two indicator bands, one purple and one yellow, will appear.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188444569975,"sku":"CLV-0274","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0274-0-cleavion-product.png?v=1781696421"},{"product_id":"2x-taq-as-pcr-master-mix-with-loading-dye-1","title":"2x Taq-AS PCR Master Mix with Loading Dye","description":"The 2xTaq-AS PCR Mix (+Dye) includes Taq-AS DNA Polymerase, reaction buffer, dNTPs, and loading dye among other components. Taq-AS (Advanced Strong) DNA Polymerase is a screened variant capable of efficiently amplifying DNA fragments up to 5 kb. It exhibits excellent inhibitor tolerance and has a threefold faster amplification speed compared to regular Taq DNA polymerase. The PCR products do not require the addition of a loading buffer and can be directly loaded for gel electrophoresis. During the electrophoresis process, two indicator bands, one purple and one yellow, will appear.","brand":"Cleavion","offers":[{"title":"100x 1 mL","offer_id":57188444701047,"sku":"CLV-0275","price":997.7,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0275-0-cleavion-product.png?v=1781696422"},{"product_id":"2x-taq-as-pcr-master-mix","title":"2x Taq-AS PCR Master Mix","description":"The 2xTaq-AS PCR Mix includes Taq-AS DNA Polymerase, reaction buffer, dNTPs, and other components. Taq-AS (Advanced Strong) DNA Polymerase is a variant obtained through screening, capable of efficiently amplifying DNA fragments up to 5 kb. It exhibits excellent inhibitor tolerance and has a threefold faster amplification speed compared to regular Taq DNA polymerase.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188444864887,"sku":"CLV-0276","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0276-0-cleavion-product.png?v=1781696425"},{"product_id":"phi29-dna-polymerase-for-rolling-circle-amplification","title":"Phi29 DNA Polymerase for Rolling Circle Amplification","description":"Phi29 II DNA Polymerase is engineered from the original phi29 DNA Polymerase with significantly improved performance. This enzyme is significantly improved over phi29 DNA polymerase in enhanced thermostability, amplification yield and sensitivity, while retaining all the benefits of the wild-type enzyme, with optimal amplification achieved at 42°C.","brand":"Cleavion","offers":[{"title":"500 U","offer_id":57188444963191,"sku":"CLV-0277","price":146.25,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0277-0-cleavion-product.png?v=1781696424"},{"product_id":"phi29-dna-polymerase-for-rolling-circle-amplification-1","title":"Phi29 DNA Polymerase for Rolling Circle Amplification","description":"Phi29 II DNA Polymerase is engineered from the original phi29 DNA Polymerase with significantly improved performance. This enzyme is significantly improved over phi29 DNA polymerase in enhanced thermostability, amplification yield and sensitivity, while retaining all the benefits of the wild-type enzyme, with optimal amplification achieved at 42°C.","brand":"Cleavion","offers":[{"title":"2500 U","offer_id":57188445127031,"sku":"CLV-0278","price":465.8,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0278-0-cleavion-product.png?v=1781696424"},{"product_id":"giv-all-in-one-master-mix-with-dsdnase","title":"GIV All-in-One Master Mix with dsDNase","description":"GIV All-in-One MasterMix (with dsDNase) is a simplified system specially designed for single-stranded cDNA synthesis. It contains all components required for the reaction, requiring only the addition of RNA template and water. The M-MLV GIV Reverse Transcriptase in the premix is a newly developed 4th-generation reverse transcriptase. It features faster reaction speed, completing the reverse transcription process within 5 minutes; stronger thermostability with an optimal reaction temperature of 55 °C; the cDNA product can reach up to 20 kb in length. Additionally, it exhibits tolerance to common inhibitors such as ethanol, isopropanol, humic acid, lithium chloride, and guanidine hydrochloride. dsDNase can specifically digest double-stranded DNA even in the presence of primers and probes, without degrading single-stranded DNA or RNA. It exhibits thermal sensitivity, enabling rapid irreversible inactivation at elevated temperatures to eliminate genomic contamination. This prevents dsDNase from damaging DNA within DNA-RNA hybrid strands during reverse transcription.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188445290871,"sku":"CLV-0279","price":360.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0279-0-cleavion-product.png?v=1781696426"},{"product_id":"2x-taq-plus-pcr-master-mix","title":"2x Taq-Plus PCR Master Mix","description":"\u003cstyle\u003e\n  .clv-seo-block {\n    --clv-navy: #06184a;\n    --clv-blue: #0b2f78;\n    --clv-cyan: #00c8f5;\n    --clv-cyan-soft: #e8fbff;\n    --clv-ice: #f6fcff;\n    --clv-text: #223047;\n    --clv-muted: #667085;\n    --clv-line: #d7edf5;\n    font-family: Arial, Helvetica, sans-serif;\n    color: var(--clv-text);\n    line-height: 1.65;\n    background: #ffffff;\n  }\n\n  .clv-seo-block * {\n    box-sizing: border-box;\n  }\n\n  .clv-seo-wrap {\n    max-width: 1180px;\n    margin: 0 auto;\n    padding: 28px 18px 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var(--clv-line);\n    border-left: 5px solid var(--clv-cyan);\n  }\n\n  .clv-cta h2 {\n    margin: 0 0 10px;\n    color: var(--clv-navy);\n  }\n\n  .clv-cta p {\n    margin: 0;\n  }\n\n  @media (max-width: 900px) {\n    .clv-grid,\n    .clv-two-col,\n    .clv-workflow {\n      grid-template-columns: 1fr;\n    }\n\n    .clv-hero,\n    .clv-section,\n    .clv-highlight,\n    .clv-cta {\n      padding: 22px;\n    }\n  }\n\u003c\/style\u003e\n\u003cdiv class=\"clv-seo-block\"\u003e\n\u003cdiv class=\"clv-seo-wrap\"\u003e\n\u003csection class=\"clv-hero\"\u003e\u003cspan class=\"clv-eyebrow\"\u003ePCR Polymerase Master Mix\u003c\/span\u003e\n\u003ch2\u003e2x Taq-Plus PCR Master Mix for Routine PCR Amplification\u003c\/h2\u003e\n\u003cp\u003eCleavion 2x Taq-Plus PCR Master Mix is a ready-to-use PCR premix designed for convenient, consistent DNA amplification. The 2x formulation combines Taq DNA Polymerase, reaction buffer, dNTPs, and optimized PCR components in one tube, reducing preparation time and helping improve repeatability across routine molecular biology workflows.\u003c\/p\u003e\n\u003cdiv class=\"clv-badges\"\u003e\n\u003cspan class=\"clv-badge\"\u003eSKU: CLV-0305\u003c\/span\u003e \u003cspan class=\"clv-badge\"\u003eFormat: 5 × 1 mL\u003c\/span\u003e \u003cspan class=\"clv-badge\"\u003eStorage: -20 °C\u003c\/span\u003e \u003cspan class=\"clv-badge\"\u003eFor PCR products up to 5 kb\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"clv-grid\"\u003e\n\u003carticle class=\"clv-card\"\u003e\n\u003ch3\u003eReady-to-use 2x PCR premix\u003c\/h3\u003e\n\u003cp\u003eThe master mix format simplifies reaction setup by reducing the number of individual reagents that must be pipetted into each PCR tube.\u003c\/p\u003e\n\u003c\/article\u003e\n\u003carticle class=\"clv-card\"\u003e\n\u003ch3\u003eOptimized for routine PCR\u003c\/h3\u003e\n\u003cp\u003eSuitable for standard endpoint PCR, colony screening, template verification, insert checking, and general DNA amplification tasks.\u003c\/p\u003e\n\u003c\/article\u003e\n\u003carticle class=\"clv-card\"\u003e\n\u003ch3\u003eDirect T\/A cloning compatibility\u003c\/h3\u003e\n\u003cp\u003ePCR products generated with Taq-based amplification can be used for downstream T\/A cloning workflows when compatible with the selected cloning system.\u003c\/p\u003e\n\u003c\/article\u003e\n\u003c\/div\u003e\n\u003csection class=\"clv-section\"\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e2x Taq-Plus PCR Master Mix is formulated for researchers who need reliable PCR amplification with fewer preparation steps. Because the mix already contains polymerase, buffer, dNTPs, and supporting reaction components, users only need to add template DNA, primers, and nuclease-free water to prepare the final PCR reaction.\u003c\/p\u003e\n\u003cp\u003eThis PCR master mix is especially useful for laboratories running repeated PCR assays, screening multiple samples, or standardizing reaction setup between users. The premixed format helps reduce pipetting variation and supports efficient setup of single reactions or larger sample batches.\u003c\/p\u003e\n\u003cdiv class=\"clv-two-col\"\u003e\n\u003cdiv class=\"clv-card\"\u003e\n\u003ch3\u003eRecommended Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine PCR amplification\u003c\/li\u003e\n\u003cli\u003eDNA fragment amplification up to 5 kb\u003c\/li\u003e\n\u003cli\u003eColony PCR and clone screening\u003c\/li\u003e\n\u003cli\u003eGenotyping PCR workflows\u003c\/li\u003e\n\u003cli\u003eTemplate confirmation and insert verification\u003c\/li\u003e\n\u003cli\u003ePreparation of PCR products for T\/A cloning\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-card\"\u003e\n\u003ch3\u003eKey Advantages\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eConvenient 2x master mix format\u003c\/li\u003e\n\u003cli\u003eReduced pipetting steps during PCR setup\u003c\/li\u003e\n\u003cli\u003eConsistent reagent composition between reactions\u003c\/li\u003e\n\u003cli\u003eGood amplification specificity for routine targets\u003c\/li\u003e\n\u003cli\u003eCompatible with common molecular biology workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-section\"\u003e\n\u003ch2\u003eTechnical Specifications\u003c\/h2\u003e\n\u003ctable class=\"clv-spec-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Name\u003c\/th\u003e\n\u003ctd\u003e2x Taq-Plus PCR Master Mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog \/ SKU\u003c\/th\u003e\n\u003ctd\u003eCLV-0305\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Type\u003c\/th\u003e\n\u003ctd\u003ePCR master mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCategory\u003c\/th\u003e\n\u003ctd\u003ePolymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSubcategory\u003c\/th\u003e\n\u003ctd\u003eConventional DNA polymerase \u0026amp; PCR mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEnzyme System\u003c\/th\u003e\n\u003ctd\u003eTaq DNA Polymerase-based PCR premix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003e2x ready-to-use liquid master mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003ctd\u003e5 × 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAmplification Range\u003c\/th\u003e\n\u003ctd\u003eDesigned for PCR products up to 5 kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage Conditions\u003c\/th\u003e\n\u003ctd\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-highlight\"\u003e\n\u003ch2\u003eHow to Use 2x Taq-Plus PCR Master Mix\u003c\/h2\u003e\n\u003cp\u003eThe 2x concentration is intended to be diluted to 1x in the final PCR reaction. In a typical workflow, combine the master mix with forward primer, reverse primer, DNA template, and nuclease-free water according to the final reaction volume required by your assay.\u003c\/p\u003e\n\u003cdiv class=\"clv-workflow\"\u003e\n\u003cdiv class=\"clv-step\"\u003e\n\u003cstrong\u003e1. Thaw \u0026amp; mix\u003c\/strong\u003e Bring the master mix to working temperature and mix gently before use.\u003c\/div\u003e\n\u003cdiv class=\"clv-step\"\u003e\n\u003cstrong\u003e2. Add primers\u003c\/strong\u003e Add forward and reverse primers according to your validated PCR design.\u003c\/div\u003e\n\u003cdiv class=\"clv-step\"\u003e\n\u003cstrong\u003e3. Add template\u003c\/strong\u003e Add DNA template and nuclease-free water to reach the final reaction volume.\u003c\/div\u003e\n\u003cdiv class=\"clv-step\"\u003e\n\u003cstrong\u003e4. Run PCR\u003c\/strong\u003e Use cycling conditions suitable for the template, primer pair, and expected amplicon length.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-section\"\u003e\n\u003ch2\u003eWhy Choose Cleavion 2x Taq-Plus PCR Master Mix?\u003c\/h2\u003e\n\u003cp\u003eCleavion 2x Taq-Plus PCR Master Mix is designed for laboratories that need a practical PCR reagent for everyday amplification tasks. The premixed format supports efficient preparation, helps reduce manual reagent handling, and allows researchers to focus on template quality, primer design, and PCR optimization.\u003c\/p\u003e\n\u003cp\u003eFor workflows such as clone screening, routine gene amplification, sample verification, and T\/A cloning preparation, this Taq-based PCR master mix offers a convenient balance of usability and performance.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-faq\"\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cdiv class=\"clv-faq-grid\"\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat is 2x Taq-Plus PCR Master Mix used for?\u003c\/h3\u003e\n\u003cp\u003eIt is used for routine PCR amplification of DNA fragments. Common applications include endpoint PCR, colony PCR, clone screening, genotyping workflows, and insert verification.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat does “2x” mean in a PCR master mix?\u003c\/h3\u003e\n\u003cp\u003e“2x” means the master mix is supplied at twice the final working concentration. It is normally combined with primers, template DNA, and water so that the final PCR reaction contains the master mix at 1x concentration.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat components are included in the master mix?\u003c\/h3\u003e\n\u003cp\u003eThe mix contains Taq DNA Polymerase, PCR reaction buffer, dNTPs, and optimized components required for standard PCR setup. Users add primers, DNA template, and nuclease-free water.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eCan PCR products from this mix be used for T\/A cloning?\u003c\/h3\u003e\n\u003cp\u003eYes. PCR products generated with this Taq-based master mix can be directly used for T\/A cloning workflows when the downstream cloning system is compatible.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat is the recommended storage temperature?\u003c\/h3\u003e\n\u003cp\u003eStore Cleavion 2x Taq-Plus PCR Master Mix at -20 °C. Avoid unnecessary freeze-thaw cycles and mix gently before use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat amplicon size can this master mix amplify?\u003c\/h3\u003e\n\u003cp\u003eThe product is designed to amplify DNA fragments up to 5 kb, depending on template quality, primer design, GC content, and PCR cycling conditions.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-cta\"\u003e\n\u003ch2\u003eCleavion PCR Reagents for Molecular Biology Workflows\u003c\/h2\u003e\n\u003cp\u003eUse 2x Taq-Plus PCR Master Mix when you need a convenient, Taq-based PCR premix for routine amplification, screening, and cloning-related workflows. Its ready-to-use 2x format helps simplify setup while supporting consistent PCR performance across repeated assays.\u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"Product\",\n  \"name\": \"2x Taq-Plus PCR Master Mix\",\n  \"sku\": \"CLV-0305\",\n  \"brand\": {\n    \"@type\": \"Brand\",\n    \"name\": \"Cleavion\"\n  },\n  \"category\": \"PCR Master Mix\",\n  \"description\": \"Cleavion 2x Taq-Plus PCR Master Mix is a ready-to-use Taq DNA Polymerase-based PCR premix containing reaction buffer, dNTPs, and optimized components for routine PCR amplification up to 5 kb.\",\n  \"url\": \"https:\/\/cleavion.com\/products\/2x-taq-plus-pcr-master-mix\",\n  \"additionalProperty\": [\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Catalog number\",\n      \"value\": \"CLV-0305\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Format\",\n      \"value\": \"2x ready-to-use PCR master mix\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Pack size\",\n      \"value\": \"5 × 1 mL\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Storage conditions\",\n      \"value\": \"-20 °C\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Application\",\n      \"value\": \"Routine PCR amplification, colony PCR, clone screening, genotyping, T\/A cloning preparation\"\n    }\n  ]\n}\n\u003c\/script\u003e\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188446011767,"sku":"CLV-0305","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0305-0-cleavion-product.png?v=1781696440"}],"url":"https:\/\/cleavion.com\/collections\/pcr-enzymes-dna-polymerases.oembed","provider":"Cleavion","version":"1.0","type":"link"}