{"title":"PCR Master Mixes","description":"\u003cp\u003eCleavion PCR Master Mixes are ready-to-use amplification reagents designed to simplify PCR setup and improve workflow consistency. The category includes 2x master mix formats, dye-containing options, high-fidelity mixes and Taq-based PCR formulations supplied in practical vial and bulk formats.\u003c\/p\u003e\u003cp\u003eThis collection is ideal for customers who want convenient PCR reagents without preparing separate polymerase, buffer, magnesium and dNTP components. Use Shopify filters for product format, pack size and mix type to separate standard PCR, high-fidelity PCR and dye-included master mixes.\u003c\/p\u003e","products":[{"product_id":"2-pcr-master-mix","title":"2× PCR Master Mix","description":"The 2x S705 HiFi Master Mix is a ready-to-use 2x premix that contains S705 High-Fidelity DNA Polymerase, dNTPs, and a optimized reaction buffer for PCR. It only requires the addition of template, primers, and Nuclease-Free Water to perform high-fidelity PCR amplification. S705 High-Fidelity DNA Polymerase is a high-fidelity DNA polymerase obtained through directed evolution.Its fidelity is 70-fold higher than that of Taq polymerase, and its an amplification speed is five times faster than that of Pfu polymerase. The 2x S705 HiFi Master Mix is formulated with unique extension factors and specificity enhancers, which significantly improves the capacity for long fragment amplification, specificity, and product yield. When using simple templates such as λDNA and plasmids, the 2x S705 HiFi Master Mix can easily amplify fragments up to 20 kb in length. When using complex templates like genomic DNA, it can effectively amplify fragments up to 12 kb. Furthermore, the 2x S705 HiFi Master Mix demonstrates good tolerance to PCR inhibitors, enabling excellent amplification even from crude templates.","brand":"Cleavion","offers":[{"title":"1 mL","offer_id":57188433985911,"sku":"CLV-0021","price":63.8,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0021-0-cleavion-product.png?v=1781696333"},{"product_id":"2-pcr-master-mix-1","title":"2× PCR Master Mix","description":"The 2x S705 HiFi Master Mix is a ready-to-use 2x premix that contains S705 High-Fidelity DNA Polymerase, dNTPs, and a optimized reaction buffer for PCR. It only requires the addition of template, primers, and Nuclease-Free Water to perform high-fidelity PCR amplification. S705 High-Fidelity DNA Polymerase is a high-fidelity DNA polymerase obtained through directed evolution.Its fidelity is 70-fold higher than that of Taq polymerase, and its an amplification speed is five times faster than that of Pfu polymerase. The 2x S705 HiFi Master Mix is formulated with unique extension factors and specificity enhancers, which significantly improves the capacity for long fragment amplification, specificity, and product yield. When using simple templates such as λDNA and plasmids, the 2x S705 HiFi Master Mix can easily amplify fragments up to 20 kb in length. When using complex templates like genomic DNA, it can effectively amplify fragments up to 12 kb. Furthermore, the 2x S705 HiFi Master Mix demonstrates good tolerance to PCR inhibitors, enabling excellent amplification even from crude templates.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188434149751,"sku":"CLV-0022","price":99.45,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0022-0-cleavion-product.png?v=1781696334"},{"product_id":"2-pcr-master-mix-2","title":"2× PCR Master Mix","description":"The 2x S705 HiFi Master Mix is a ready-to-use 2x premix that contains S705 High-Fidelity DNA Polymerase, dNTPs, and a optimized reaction buffer for PCR. It only requires the addition of template, primers, and Nuclease-Free Water to perform high-fidelity PCR amplification. S705 High-Fidelity DNA Polymerase is a high-fidelity DNA polymerase obtained through directed evolution.Its fidelity is 70-fold higher than that of Taq polymerase, and its an amplification speed is five times faster than that of Pfu polymerase. The 2x S705 HiFi Master Mix is formulated with unique extension factors and specificity enhancers, which significantly improves the capacity for long fragment amplification, specificity, and product yield. When using simple templates such as λDNA and plasmids, the 2x S705 HiFi Master Mix can easily amplify fragments up to 20 kb in length. When using complex templates like genomic DNA, it can effectively amplify fragments up to 12 kb. Furthermore, the 2x S705 HiFi Master Mix demonstrates good tolerance to PCR inhibitors, enabling excellent amplification even from crude templates.","brand":"Cleavion","offers":[{"title":"20x 1 mL","offer_id":57188434411895,"sku":"CLV-0023","price":316.14,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0023-0-cleavion-product.png?v=1781696335"},{"product_id":"2-pcr-master-mix-with-dye","title":"2× PCR Master Mix (with Dye)","description":"The 2x S705 HiFi Master Mix (+Dye) is a ready-to-use 2x premix that contains S705 High-Fidelity DNA Polymerase, dNTPs, and a optimized reaction buffer for PCR. It only requires the addition of template, primers, and Nuclease-Free Water to perform high-fidelity PCR amplification. S705 High-Fidelity DNA Polymerase is a high-fidelity DNA polymerase obtained through directed evolution.Its fidelity is 70-fold higher than that of Taq polymerase, and its an amplification speed is five times faster than that of Pfu polymerase. The 2x S705 HiFi Master Mix (+Dye) is formulated with unique extension factors and specificity enhancers, which significantly improves the capacity for long fragment amplification, specificity, and product yield. When using simple templates such as λDNA and plasmids, the 2x S705 HiFi Master Mix (+Dye) can easily amplify fragments up to 20 kb in length. When using complex templates like genomic DNA, it can effectively amplify fragments up to 12 kb. Furthermore, the 2x S705 HiFi Master Mix (+Dye) demonstrates good tolerance to PCR inhibitors, enabling excellent amplification even from crude templates. The premix contains red and yellow tracking dyes and PCR products can be directly loaded for electrophoresis without adding a Loading Buffer. In 1% agarose gel, the red dye has a migration rate comparable to that of the 1500 bp dsDNA fragment and the yellow dye has a migration rate comparable to that of the 10 bp dsDNA fragment.","brand":"Cleavion","offers":[{"title":"1 mL","offer_id":57188434575735,"sku":"CLV-0024","price":63.8,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0024-0-cleavion-product.png?v=1781696336"},{"product_id":"2-pcr-master-mix-with-dye-1","title":"2× PCR Master Mix (with Dye)","description":"The 2x S705 HiFi Master Mix (+Dye) is a ready-to-use 2x premix that contains S705 High-Fidelity DNA Polymerase, dNTPs, and a optimized reaction buffer for PCR. It only requires the addition of template, primers, and Nuclease-Free Water to perform high-fidelity PCR amplification. S705 High-Fidelity DNA Polymerase is a high-fidelity DNA polymerase obtained through directed evolution.Its fidelity is 70-fold higher than that of Taq polymerase, and its an amplification speed is five times faster than that of Pfu polymerase. The 2x S705 HiFi Master Mix (+Dye) is formulated with unique extension factors and specificity enhancers, which significantly improves the capacity for long fragment amplification, specificity, and product yield. When using simple templates such as λDNA and plasmids, the 2x S705 HiFi Master Mix (+Dye) can easily amplify fragments up to 20 kb in length. When using complex templates like genomic DNA, it can effectively amplify fragments up to 12 kb. Furthermore, the 2x S705 HiFi Master Mix (+Dye) demonstrates good tolerance to PCR inhibitors, enabling excellent amplification even from crude templates. The premix contains red and yellow tracking dyes and PCR products can be directly loaded for electrophoresis without adding a Loading Buffer. In 1% agarose gel, the red dye has a migration rate comparable to that of the 1500 bp dsDNA fragment and the yellow dye has a migration rate comparable to that of the 10 bp dsDNA fragment.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188434739575,"sku":"CLV-0025","price":99.45,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0025-0-cleavion-product.png?v=1781696336"},{"product_id":"2-pcr-master-mix-with-dye-2","title":"2× PCR Master Mix (with Dye)","description":"The 2x S705 HiFi Master Mix (+Dye) is a ready-to-use 2x premix that contains S705 High-Fidelity DNA Polymerase, dNTPs, and a optimized reaction buffer for PCR. It only requires the addition of template, primers, and Nuclease-Free Water to perform high-fidelity PCR amplification. S705 High-Fidelity DNA Polymerase is a high-fidelity DNA polymerase obtained through directed evolution.Its fidelity is 70-fold higher than that of Taq polymerase, and its an amplification speed is five times faster than that of Pfu polymerase. The 2x S705 HiFi Master Mix (+Dye) is formulated with unique extension factors and specificity enhancers, which significantly improves the capacity for long fragment amplification, specificity, and product yield. When using simple templates such as λDNA and plasmids, the 2x S705 HiFi Master Mix (+Dye) can easily amplify fragments up to 20 kb in length. When using complex templates like genomic DNA, it can effectively amplify fragments up to 12 kb. Furthermore, the 2x S705 HiFi Master Mix (+Dye) demonstrates good tolerance to PCR inhibitors, enabling excellent amplification even from crude templates. The premix contains red and yellow tracking dyes and PCR products can be directly loaded for electrophoresis without adding a Loading Buffer. In 1% agarose gel, the red dye has a migration rate comparable to that of the 1500 bp dsDNA fragment and the yellow dye has a migration rate comparable to that of the 10 bp dsDNA fragment.","brand":"Cleavion","offers":[{"title":"20x 1 mL","offer_id":57188434870647,"sku":"CLV-0026","price":316.14,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0026-0-cleavion-product.png?v=1781696337"},{"product_id":"sybr-qpcr-mix-universal","title":"SYBR qPCR Mix (Universal)","description":"F488 SYBR qPCR Mix (Universal) is a special premix for qPCR detection using SYBR Green I chimeric fluorescence method. The Mix contains all components for qPCR, except DNA templates and primers. The core component is the updated hot-start Taq DNA polymerase with better specificity. With the optimized buffer, the mix is able to obtain consistent and reliable qPCR results in a broad template concentration range. This product contains universial reference dye compatible with most of qPCR devices, and do not require additional ROX dyes before reaction.","brand":"Cleavion","offers":[{"title":"25x 1 mL","offer_id":57188435100023,"sku":"CLV-0046","price":157.14,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0046-0-cleavion-product.png?v=1781696338"},{"product_id":"2-taq-pcr-mix","title":"2× Taq PCR Mix","description":"Taq PCR Aurora Mix is a ready-to-use PCR mix with the concentration of 2x. lt offers convenience and reduces the risk of contamination. PCR products synthesized with this mix have an overhang of A bases at the 3' end, enabling direct T\/A cloning after purification. The Mix contains Taq DNA polymerase and a protein with 3'→5' exonuclease activity, enabling efficient amplification of DNA fragments up to 7 kb with high yields. Coupled with the optimized reaction buffer, it can achieve efficient amplification for different GC contents (ranging from 30% to 70%) . In addition, compared with WT-Taq DNA polymerase, the extension rate of Taq PCR Aurora Mix is enhanced by 2 to 4 times, which can effectively shorten the reaction time. This PCR Mix contains two dyes. The PCR products can be directly loaded for electrophoresis without adding a Loading Buffer, and two indicator bands in blue and red colors will appear during the electrophoresis process. These dyes do not interfere with the efficiency of PCR amplification. However, for experiments that require optical analyses such as absorbance and fluorescence of the PCR products, it is recommended to purify the PCR products prior to analysis.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188435296631,"sku":"CLV-0029","price":59.86,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0029-0-cleavion-product.png?v=1781696339"},{"product_id":"2-taq-pcr-mix-1","title":"2× Taq PCR Mix","description":"Taq PCR Aurora Mix is a ready-to-use PCR mix with the concentration of 2x. lt offers convenience and reduces the risk of contamination. PCR products synthesized with this mix have an overhang of A bases at the 3' end, enabling direct T\/A cloning after purification. The Mix contains Taq DNA polymerase and a protein with 3'→5' exonuclease activity, enabling efficient amplification of DNA fragments up to 7 kb with high yields. Coupled with the optimized reaction buffer, it can achieve efficient amplification for different GC contents (ranging from 30% to 70%) . In addition, compared with WT-Taq DNA polymerase, the extension rate of Taq PCR Aurora Mix is enhanced by 2 to 4 times, which can effectively shorten the reaction time. This PCR Mix contains two dyes. The PCR products can be directly loaded for electrophoresis without adding a Loading Buffer, and two indicator bands in blue and red colors will appear during the electrophoresis process. These dyes do not interfere with the efficiency of PCR amplification. However, for experiments that require optical analyses such as absorbance and fluorescence of the PCR products, it is recommended to purify the PCR products prior to analysis.","brand":"Cleavion","offers":[{"title":"100x 1 mL","offer_id":57188435460471,"sku":"CLV-0030","price":194.64,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0030-0-cleavion-product.png?v=1781696340"},{"product_id":"taq-plus-pcr-mix-2","title":"Taq-Plus PCR Mix (2×)","description":"Taq-Plus PCR Forest Mix (2x) V2 is a pre-mixed PCR reaction solution formulated with Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. It is capable of robust amplification of up to 6 kb from genomic DNA. PCR products amplified with this mix have a 3'-terminal adenosine (A), and therefore PCR products can be used directly for T\/A cloning. The Forest mix is supplemented with two tracking dyes that allows for direct loading of the PCR product on a gel. The dyes in the mix do not interfere with PCR performance but need to purify the PCR product when it's used for absorbance, fluorescence, etc. In addition, the dye-free Taq-Plus PCR Master Mix (2x) V2 can also be used.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188435558775,"sku":"CLV-0031","price":58.28,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0031-0-cleavion-product.png?v=1781696341"},{"product_id":"taq-plus-pcr-mix-3","title":"Taq-Plus PCR Mix (2×)","description":"Taq-Plus PCR Forest Mix (2x) V2 is a pre-mixed PCR reaction solution formulated with Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. It is capable of robust amplification of up to 6 kb from genomic DNA. PCR products amplified with this mix have a 3'-terminal adenosine (A), and therefore PCR products can be used directly for T\/A cloning. The Forest mix is supplemented with two tracking dyes that allows for direct loading of the PCR product on a gel. The dyes in the mix do not interfere with PCR performance but need to purify the PCR product when it's used for absorbance, fluorescence, etc. In addition, the dye-free Taq-Plus PCR Master Mix (2x) V2 can also be used.","brand":"Cleavion","offers":[{"title":"100x 1 mL","offer_id":57188435657079,"sku":"CLV-0032","price":239.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0032-0-cleavion-product.png?v=1781696341"},{"product_id":"all-in-one-first-strand-synthesis-master-mix-with-dsdnase","title":"All-in-One First-Strand Synthesis Master Mix (with dsDNase)","description":"The All-in-One First-Strand Synthesis MasterMix (with dsDNase) is a comprehensive kit for first-strand cDNA synthesis, containing reverse transcriptase, reaction buffer, RNAse inhibitor, dNTPs, Oligo (dT) 20VN, and random primers. It provides all the necessary components for efficient cDNA synthesis and requires only the addition of RNA template and water to initiate the reaction. Within just 15 minutes, cDNA fragments up to 12 kb in length can be obtained, which can be utilized downstream in experiments such as qPCR and conventional PCR. This kit incorporates dsDNase, which effectively removes genomic DNA contamination. Unlike conventional DNase I, dsDNase specifically digests double-stranded DNA (dsDNA) and hybrid DNA-RNA molecules while exhibiting thermal sensitivity, enabling rapid and irreversible inactivation under high-temperature conditions. Compared to traditional methods that employ DNase I to remove genomic DNA contamination, the use of dsDNase eliminates the need for additional EDTA for inactivation. This not only saves experimental time but also reduces inhibition of the reverse transcription reaction.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188436115831,"sku":"CLV-0035","price":83.35,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0035-0-cleavion-product.png?v=1781696343"},{"product_id":"all-in-one-first-strand-synthesis-master-mix","title":"All-in-One First-Strand Synthesis Master Mix","description":"All-in-One First-Strand Synthesis MasterMix is a high-quality, efficient, and convenient one-step cDNA synthesis mix. It is designed to minimize contamination and contains all the necessary components for first-strand cDNA synthesis, including thermostable M-MLV GIII Reverse Transcriptase and its reaction buffer, RNase inhibitor, dNTPs, Oligo (dT) 20VN, and random primers-all the necessary components. Simply add RNA template and water to initiate the reaction. Using the reverse transcription pre-mix, cDNA of up to 12 kb in size can be obtained within 15 minutes.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188436246903,"sku":"CLV-0036","price":86.8,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0036-0-cleavion-product.png?v=1781696344"},{"product_id":"2x-taq-as-pcr-master-mix-with-loading-dye","title":"2x Taq-AS PCR Master Mix with Loading Dye","description":"The 2xTaq-AS PCR Mix (+Dye) includes Taq-AS DNA Polymerase, reaction buffer, dNTPs, and loading dye among other components. Taq-AS (Advanced Strong) DNA Polymerase is a screened variant capable of efficiently amplifying DNA fragments up to 5 kb. It exhibits excellent inhibitor tolerance and has a threefold faster amplification speed compared to regular Taq DNA polymerase. The PCR products do not require the addition of a loading buffer and can be directly loaded for gel electrophoresis. During the electrophoresis process, two indicator bands, one purple and one yellow, will appear.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188444569975,"sku":"CLV-0274","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0274-0-cleavion-product.png?v=1781696421"},{"product_id":"2x-taq-as-pcr-master-mix-with-loading-dye-1","title":"2x Taq-AS PCR Master Mix with Loading Dye","description":"The 2xTaq-AS PCR Mix (+Dye) includes Taq-AS DNA Polymerase, reaction buffer, dNTPs, and loading dye among other components. Taq-AS (Advanced Strong) DNA Polymerase is a screened variant capable of efficiently amplifying DNA fragments up to 5 kb. It exhibits excellent inhibitor tolerance and has a threefold faster amplification speed compared to regular Taq DNA polymerase. The PCR products do not require the addition of a loading buffer and can be directly loaded for gel electrophoresis. During the electrophoresis process, two indicator bands, one purple and one yellow, will appear.","brand":"Cleavion","offers":[{"title":"100x 1 mL","offer_id":57188444701047,"sku":"CLV-0275","price":997.7,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0275-0-cleavion-product.png?v=1781696422"},{"product_id":"2x-taq-as-pcr-master-mix","title":"2x Taq-AS PCR Master Mix","description":"The 2xTaq-AS PCR Mix includes Taq-AS DNA Polymerase, reaction buffer, dNTPs, and other components. Taq-AS (Advanced Strong) DNA Polymerase is a variant obtained through screening, capable of efficiently amplifying DNA fragments up to 5 kb. It exhibits excellent inhibitor tolerance and has a threefold faster amplification speed compared to regular Taq DNA polymerase.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188444864887,"sku":"CLV-0276","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0276-0-cleavion-product.png?v=1781696425"},{"product_id":"giv-all-in-one-master-mix-with-dsdnase","title":"GIV All-in-One Master Mix with dsDNase","description":"GIV All-in-One MasterMix (with dsDNase) is a simplified system specially designed for single-stranded cDNA synthesis. It contains all components required for the reaction, requiring only the addition of RNA template and water. The M-MLV GIV Reverse Transcriptase in the premix is a newly developed 4th-generation reverse transcriptase. It features faster reaction speed, completing the reverse transcription process within 5 minutes; stronger thermostability with an optimal reaction temperature of 55 °C; the cDNA product can reach up to 20 kb in length. Additionally, it exhibits tolerance to common inhibitors such as ethanol, isopropanol, humic acid, lithium chloride, and guanidine hydrochloride. dsDNase can specifically digest double-stranded DNA even in the presence of primers and probes, without degrading single-stranded DNA or RNA. It exhibits thermal sensitivity, enabling rapid irreversible inactivation at elevated temperatures to eliminate genomic contamination. This prevents dsDNase from damaging DNA within DNA-RNA hybrid strands during reverse transcription.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188445290871,"sku":"CLV-0279","price":360.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0279-0-cleavion-product.png?v=1781696426"},{"product_id":"2x-taq-plus-pcr-master-mix","title":"2x Taq-Plus PCR Master Mix","description":"\u003cstyle\u003e\n  .clv-seo-block {\n    --clv-navy: #06184a;\n    --clv-blue: #0b2f78;\n    --clv-cyan: #00c8f5;\n    --clv-cyan-soft: #e8fbff;\n    --clv-ice: #f6fcff;\n    --clv-text: #223047;\n    --clv-muted: #667085;\n    --clv-line: #d7edf5;\n    font-family: Arial, Helvetica, sans-serif;\n    color: var(--clv-text);\n    line-height: 1.65;\n    background: #ffffff;\n  }\n\n  .clv-seo-block * {\n    box-sizing: border-box;\n  }\n\n  .clv-seo-wrap {\n    max-width: 1180px;\n    margin: 0 auto;\n    padding: 28px 18px 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255, 255, 0.08);\n    border: 1px solid rgba(0, 200, 245, 0.25);\n    padding: 18px;\n  }\n\n  .clv-step strong {\n    display: block;\n    color: #86efff;\n    margin-bottom: 6px;\n  }\n\n  .clv-faq {\n    margin-top: 24px;\n  }\n\n  .clv-faq h2 {\n    color: var(--clv-navy);\n    font-size: 30px;\n    margin: 0 0 14px;\n  }\n\n  .clv-faq-grid {\n    display: grid;\n    grid-template-columns: 1fr;\n    gap: 12px;\n  }\n\n  .clv-faq-item {\n    background: #ffffff;\n    border: 1px solid var(--clv-line);\n    padding: 18px;\n    box-shadow: 0 8px 22px rgba(6, 24, 74, 0.05);\n    border-left: 4px solid var(--clv-cyan);\n  }\n\n  .clv-faq-item h3 {\n    margin: 0 0 8px;\n    color: var(--clv-navy);\n    font-size: 18px;\n    line-height: 1.35;\n  }\n\n  .clv-faq-item p {\n    margin: 0;\n    color: var(--clv-text);\n  }\n\n  .clv-cta {\n    margin-top: 24px;\n    padding: 26px;\n    background: linear-gradient(135deg, var(--clv-cyan-soft), #ffffff);\n    border: 1px solid var(--clv-line);\n    border-left: 5px solid var(--clv-cyan);\n  }\n\n  .clv-cta h2 {\n    margin: 0 0 10px;\n    color: var(--clv-navy);\n  }\n\n  .clv-cta p {\n    margin: 0;\n  }\n\n  @media (max-width: 900px) {\n    .clv-grid,\n    .clv-two-col,\n    .clv-workflow {\n      grid-template-columns: 1fr;\n    }\n\n    .clv-hero,\n    .clv-section,\n    .clv-highlight,\n    .clv-cta {\n      padding: 22px;\n    }\n  }\n\u003c\/style\u003e\n\u003cdiv class=\"clv-seo-block\"\u003e\n\u003cdiv class=\"clv-seo-wrap\"\u003e\n\u003csection class=\"clv-hero\"\u003e\u003cspan class=\"clv-eyebrow\"\u003ePCR Polymerase Master Mix\u003c\/span\u003e\n\u003ch2\u003e2x Taq-Plus PCR Master Mix for Routine PCR Amplification\u003c\/h2\u003e\n\u003cp\u003eCleavion 2x Taq-Plus PCR Master Mix is a ready-to-use PCR premix designed for convenient, consistent DNA amplification. The 2x formulation combines Taq DNA Polymerase, reaction buffer, dNTPs, and optimized PCR components in one tube, reducing preparation time and helping improve repeatability across routine molecular biology workflows.\u003c\/p\u003e\n\u003cdiv class=\"clv-badges\"\u003e\n\u003cspan class=\"clv-badge\"\u003eSKU: CLV-0305\u003c\/span\u003e \u003cspan class=\"clv-badge\"\u003eFormat: 5 × 1 mL\u003c\/span\u003e \u003cspan class=\"clv-badge\"\u003eStorage: -20 °C\u003c\/span\u003e \u003cspan class=\"clv-badge\"\u003eFor PCR products up to 5 kb\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"clv-grid\"\u003e\n\u003carticle class=\"clv-card\"\u003e\n\u003ch3\u003eReady-to-use 2x PCR premix\u003c\/h3\u003e\n\u003cp\u003eThe master mix format simplifies reaction setup by reducing the number of individual reagents that must be pipetted into each PCR tube.\u003c\/p\u003e\n\u003c\/article\u003e\n\u003carticle class=\"clv-card\"\u003e\n\u003ch3\u003eOptimized for routine PCR\u003c\/h3\u003e\n\u003cp\u003eSuitable for standard endpoint PCR, colony screening, template verification, insert checking, and general DNA amplification tasks.\u003c\/p\u003e\n\u003c\/article\u003e\n\u003carticle class=\"clv-card\"\u003e\n\u003ch3\u003eDirect T\/A cloning compatibility\u003c\/h3\u003e\n\u003cp\u003ePCR products generated with Taq-based amplification can be used for downstream T\/A cloning workflows when compatible with the selected cloning system.\u003c\/p\u003e\n\u003c\/article\u003e\n\u003c\/div\u003e\n\u003csection class=\"clv-section\"\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e2x Taq-Plus PCR Master Mix is formulated for researchers who need reliable PCR amplification with fewer preparation steps. Because the mix already contains polymerase, buffer, dNTPs, and supporting reaction components, users only need to add template DNA, primers, and nuclease-free water to prepare the final PCR reaction.\u003c\/p\u003e\n\u003cp\u003eThis PCR master mix is especially useful for laboratories running repeated PCR assays, screening multiple samples, or standardizing reaction setup between users. The premixed format helps reduce pipetting variation and supports efficient setup of single reactions or larger sample batches.\u003c\/p\u003e\n\u003cdiv class=\"clv-two-col\"\u003e\n\u003cdiv class=\"clv-card\"\u003e\n\u003ch3\u003eRecommended Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine PCR amplification\u003c\/li\u003e\n\u003cli\u003eDNA fragment amplification up to 5 kb\u003c\/li\u003e\n\u003cli\u003eColony PCR and clone screening\u003c\/li\u003e\n\u003cli\u003eGenotyping PCR workflows\u003c\/li\u003e\n\u003cli\u003eTemplate confirmation and insert verification\u003c\/li\u003e\n\u003cli\u003ePreparation of PCR products for T\/A cloning\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-card\"\u003e\n\u003ch3\u003eKey Advantages\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eConvenient 2x master mix format\u003c\/li\u003e\n\u003cli\u003eReduced pipetting steps during PCR setup\u003c\/li\u003e\n\u003cli\u003eConsistent reagent composition between reactions\u003c\/li\u003e\n\u003cli\u003eGood amplification specificity for routine targets\u003c\/li\u003e\n\u003cli\u003eCompatible with common molecular biology workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-section\"\u003e\n\u003ch2\u003eTechnical Specifications\u003c\/h2\u003e\n\u003ctable class=\"clv-spec-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Name\u003c\/th\u003e\n\u003ctd\u003e2x Taq-Plus PCR Master Mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog \/ SKU\u003c\/th\u003e\n\u003ctd\u003eCLV-0305\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Type\u003c\/th\u003e\n\u003ctd\u003ePCR master mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCategory\u003c\/th\u003e\n\u003ctd\u003ePolymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSubcategory\u003c\/th\u003e\n\u003ctd\u003eConventional DNA polymerase \u0026amp; PCR mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEnzyme System\u003c\/th\u003e\n\u003ctd\u003eTaq DNA Polymerase-based PCR premix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003e2x ready-to-use liquid master mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003ctd\u003e5 × 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAmplification Range\u003c\/th\u003e\n\u003ctd\u003eDesigned for PCR products up to 5 kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage Conditions\u003c\/th\u003e\n\u003ctd\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-highlight\"\u003e\n\u003ch2\u003eHow to Use 2x Taq-Plus PCR Master Mix\u003c\/h2\u003e\n\u003cp\u003eThe 2x concentration is intended to be diluted to 1x in the final PCR reaction. In a typical workflow, combine the master mix with forward primer, reverse primer, DNA template, and nuclease-free water according to the final reaction volume required by your assay.\u003c\/p\u003e\n\u003cdiv class=\"clv-workflow\"\u003e\n\u003cdiv class=\"clv-step\"\u003e\n\u003cstrong\u003e1. Thaw \u0026amp; mix\u003c\/strong\u003e Bring the master mix to working temperature and mix gently before use.\u003c\/div\u003e\n\u003cdiv class=\"clv-step\"\u003e\n\u003cstrong\u003e2. Add primers\u003c\/strong\u003e Add forward and reverse primers according to your validated PCR design.\u003c\/div\u003e\n\u003cdiv class=\"clv-step\"\u003e\n\u003cstrong\u003e3. Add template\u003c\/strong\u003e Add DNA template and nuclease-free water to reach the final reaction volume.\u003c\/div\u003e\n\u003cdiv class=\"clv-step\"\u003e\n\u003cstrong\u003e4. Run PCR\u003c\/strong\u003e Use cycling conditions suitable for the template, primer pair, and expected amplicon length.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-section\"\u003e\n\u003ch2\u003eWhy Choose Cleavion 2x Taq-Plus PCR Master Mix?\u003c\/h2\u003e\n\u003cp\u003eCleavion 2x Taq-Plus PCR Master Mix is designed for laboratories that need a practical PCR reagent for everyday amplification tasks. The premixed format supports efficient preparation, helps reduce manual reagent handling, and allows researchers to focus on template quality, primer design, and PCR optimization.\u003c\/p\u003e\n\u003cp\u003eFor workflows such as clone screening, routine gene amplification, sample verification, and T\/A cloning preparation, this Taq-based PCR master mix offers a convenient balance of usability and performance.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-faq\"\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cdiv class=\"clv-faq-grid\"\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat is 2x Taq-Plus PCR Master Mix used for?\u003c\/h3\u003e\n\u003cp\u003eIt is used for routine PCR amplification of DNA fragments. Common applications include endpoint PCR, colony PCR, clone screening, genotyping workflows, and insert verification.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat does “2x” mean in a PCR master mix?\u003c\/h3\u003e\n\u003cp\u003e“2x” means the master mix is supplied at twice the final working concentration. It is normally combined with primers, template DNA, and water so that the final PCR reaction contains the master mix at 1x concentration.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat components are included in the master mix?\u003c\/h3\u003e\n\u003cp\u003eThe mix contains Taq DNA Polymerase, PCR reaction buffer, dNTPs, and optimized components required for standard PCR setup. Users add primers, DNA template, and nuclease-free water.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eCan PCR products from this mix be used for T\/A cloning?\u003c\/h3\u003e\n\u003cp\u003eYes. PCR products generated with this Taq-based master mix can be directly used for T\/A cloning workflows when the downstream cloning system is compatible.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat is the recommended storage temperature?\u003c\/h3\u003e\n\u003cp\u003eStore Cleavion 2x Taq-Plus PCR Master Mix at -20 °C. Avoid unnecessary freeze-thaw cycles and mix gently before use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat amplicon size can this master mix amplify?\u003c\/h3\u003e\n\u003cp\u003eThe product is designed to amplify DNA fragments up to 5 kb, depending on template quality, primer design, GC content, and PCR cycling conditions.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-cta\"\u003e\n\u003ch2\u003eCleavion PCR Reagents for Molecular Biology Workflows\u003c\/h2\u003e\n\u003cp\u003eUse 2x Taq-Plus PCR Master Mix when you need a convenient, Taq-based PCR premix for routine amplification, screening, and cloning-related workflows. Its ready-to-use 2x format helps simplify setup while supporting consistent PCR performance across repeated assays.\u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"Product\",\n  \"name\": \"2x Taq-Plus PCR Master Mix\",\n  \"sku\": \"CLV-0305\",\n  \"brand\": {\n    \"@type\": \"Brand\",\n    \"name\": \"Cleavion\"\n  },\n  \"category\": \"PCR Master Mix\",\n  \"description\": \"Cleavion 2x Taq-Plus PCR Master Mix is a ready-to-use Taq DNA Polymerase-based PCR premix containing reaction buffer, dNTPs, and optimized components for routine PCR amplification up to 5 kb.\",\n  \"url\": \"https:\/\/cleavion.com\/products\/2x-taq-plus-pcr-master-mix\",\n  \"additionalProperty\": [\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Catalog number\",\n      \"value\": \"CLV-0305\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Format\",\n      \"value\": \"2x ready-to-use PCR master mix\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Pack size\",\n      \"value\": \"5 × 1 mL\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Storage conditions\",\n      \"value\": \"-20 °C\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Application\",\n      \"value\": \"Routine PCR amplification, colony PCR, clone screening, genotyping, T\/A cloning preparation\"\n    }\n  ]\n}\n\u003c\/script\u003e\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188446011767,"sku":"CLV-0305","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0305-0-cleavion-product.png?v=1781696440"}],"url":"https:\/\/cleavion.com\/collections\/pcr-master-mixes.oembed","provider":"Cleavion","version":"1.0","type":"link"}