{"title":"PCR, qPCR \u0026 Reverse Transcription","description":"\u003cp\u003eDiscover our comprehensive range of PCR, qPCR, and reverse transcription reagents, including master mixes, high-fidelity DNA polymerases, SYBR Green and probe-based qPCR premixes, and cDNA synthesis kits. Optimized for sensitivity, specificity, and ease of use, our products support a wide range of amplification workflows from routine endpoint PCR to quantitative gene expression analysis and one-step RT-qPCR.\u003c\/p\u003e\u003cp\u003eWhether you're amplifying complex templates, quantifying low-abundance transcripts, or synthesizing first-strand cDNA from RNA, our reagents deliver consistent performance across sample types and instrument platforms. All products are rigorously validated to ensure reliable results, making them the trusted choice for researchers in genomics, diagnostics, and molecular biology.\u003c\/p\u003e","products":[{"product_id":"taq-hs-probe-qpcr-premix-universal","title":"Taq-HS Probe qPCR Premix (Universal)","description":"Taq-HS Probe qPCR Premix is a 2x pre-mixed solution developed for real-time quantitative PCR based on the unique “double-block”hot-start Taq DNA polymerase. This product contains all the fluorescent quantitative PCR components except for primers and sample DNA, reducing operational steps, shortening the time, and lowering the risk of contamination. lt is ideal for TaqMan probe-based detection using cDNA or DNA as the template. This product contains universial correction dye that are compatible with all of qPCR devices, including instruments that require ROX calibration, and do not require additional dyes to correct the instrument.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188435427703,"sku":"CLV-0048","price":89.43,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0048-0-cleavion-product.png?v=1781696340"},{"product_id":"one-step-rt-qpcr-probe-kit","title":"One Step RT-qPCR Probe Kit","description":"One Step RT-qPCR Probe Kit v2 is a kit for multiple reverse transcription - fluorescent quantitative PCR reactions using RNA as a template. During the experiment, reverse transcription and quantitative PCR are performed in the same reaction tube, which simplifies the experimental operation and reduces the risk of contamination. The kit contains thermo-stable Reverse Transcriptase to efficiently synthesize the first-strand cDNA, and contain hot-start Ab-Taq DNA Polymerase to quantitative amplification, making it suitable for probe based qPCR. The product already includes all components for reverse transcription - fluorescent quantitative PCR, excluding primers and sample RNA, including RNase inhibitors, RTase, and Ab-Taq. It can reduce operation steps, shorten sample addition time, and lower the risk of contamination.","brand":"Cleavion","offers":[{"title":"250 Reactions","offer_id":57188435591543,"sku":"CLV-0049","price":164.29,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0049-0-cleavion-product.png?v=1781696341"},{"product_id":"taq-sybr-qpcr-premix-universal","title":"Taq SYBR qPCR Premix (Universal)","description":"Taq Visual SYBR qPCR Premix (Universal) is a qPCR reagent specifically designed for SYBR Green I intercalating dye-based assays. This 2x premix includes all necessary qPCR components except for primers and DNA samples, thereby reducing operational steps, shortening sample loading time, and minimizing the risk of contamination. Its core component is an antibody-modified hot-start Taq DNA polymerase, combined with a meticulously optimized buffer system and PCR reaction enhancers. This formulation provides strong specificity and high amplification efficiency, effectively inhibiting non-specific amplification and enabling accurate quantification of templates across a wide concentration range, resulting in stable and reliable qPCR results. This product already contains a universal reference dye and is compatible with most qPCR instruments, eliminating the need for additional dyes for instrument calibration. This product utilizes the color-changing effect resulting from the mixing of different dyes to track the process, thereby significantly minimizing pipetting errors. Taq Visual SYBR qPCR Premix contains a blue dye, while the 10x Dilution Buffer contains a yellow dye. When Taq Visual SYBR qPCR Premix (blue) is mixed with template diluted in Dilution Buffer (yellow), a color change from blue to green occurs, allowing for an accurate visual confirmation of template addition based on the liquid color","brand":"Cleavion","offers":[{"title":"4x 1.25 mL","offer_id":57188435722615,"sku":"CLV-0050","price":68.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0050-0-cleavion-product.png?v=1781696341"},{"product_id":"taq-sybr-qpcr-premix-universal-1","title":"Taq SYBR qPCR Premix (Universal)","description":"","brand":"Cleavion","offers":[{"title":"20x 1.25 mL","offer_id":57188435886455,"sku":"CLV-0051","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0051-0-cleavion-product.png?v=1781696342"},{"product_id":"all-in-one-first-strand-synthesis-master-mix-with-dsdnase","title":"All-in-One First-Strand Synthesis Master Mix (with dsDNase)","description":"The All-in-One First-Strand Synthesis MasterMix (with dsDNase) is a comprehensive kit for first-strand cDNA synthesis, containing reverse transcriptase, reaction buffer, RNAse inhibitor, dNTPs, Oligo (dT) 20VN, and random primers. It provides all the necessary components for efficient cDNA synthesis and requires only the addition of RNA template and water to initiate the reaction. Within just 15 minutes, cDNA fragments up to 12 kb in length can be obtained, which can be utilized downstream in experiments such as qPCR and conventional PCR. This kit incorporates dsDNase, which effectively removes genomic DNA contamination. Unlike conventional DNase I, dsDNase specifically digests double-stranded DNA (dsDNA) and hybrid DNA-RNA molecules while exhibiting thermal sensitivity, enabling rapid and irreversible inactivation under high-temperature conditions. Compared to traditional methods that employ DNase I to remove genomic DNA contamination, the use of dsDNase eliminates the need for additional EDTA for inactivation. This not only saves experimental time but also reduces inhibition of the reverse transcription reaction.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188436115831,"sku":"CLV-0035","price":83.35,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0035-0-cleavion-product.png?v=1781696343"},{"product_id":"all-in-one-first-strand-synthesis-master-mix","title":"All-in-One First-Strand Synthesis Master Mix","description":"All-in-One First-Strand Synthesis MasterMix is a high-quality, efficient, and convenient one-step cDNA synthesis mix. It is designed to minimize contamination and contains all the necessary components for first-strand cDNA synthesis, including thermostable M-MLV GIII Reverse Transcriptase and its reaction buffer, RNase inhibitor, dNTPs, Oligo (dT) 20VN, and random primers-all the necessary components. Simply add RNA template and water to initiate the reaction. Using the reverse transcription pre-mix, cDNA of up to 12 kb in size can be obtained within 15 minutes.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188436246903,"sku":"CLV-0036","price":86.8,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0036-0-cleavion-product.png?v=1781696344"},{"product_id":"high-accuracy-reverse-transcriptase","title":"High-Accuracy Reverse Transcriptase","description":"The High-Accuracy Reverse Transcriptase is a modified version of M-MLV GIII Reverse Transcriptase that utilizes nucleic acid aptamer technology. It is covalently linked to the M-MLV enzyme and exhibits suppressed activity below 31°C, thereby enhancing the specificity of the enzyme's products. The optimal reaction temperature for this enzyme is 50°C, and it possesses enhanced extension capabilities, making it suitable for synthesizing longer cDNA fragments.","brand":"Cleavion","offers":[{"title":"10000 U","offer_id":57188436345207,"sku":"CLV-0037","price":157.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0037-0-cleavion-product.png?v=1781696344"},{"product_id":"rtase-iii-primer-flexible-all-in-one-mix-with-dsdnase","title":"RTase III Primer Flexible All-in-One Mix (with dsDNase)","description":"RTase III Primer Flexible All-in-One Mix is a high-quality, efficient, and convenient one-step cDNA synthesis premix. It is designed to minimize contamination and contains all the necessary components for first-strand cDNA synthesis, including M-MLV GIII Reverse Transcriptase and its reaction buffer, RNase inhibitor, dNTPs, and random primers-all the necessary components. Additionally, it requires the addition of RNA templates, primers, and water to initiate the reaction. This kit can be used with different types of reverse transcription primers to meet diverse experimental needs. Depending on the experimental design, Oligo (dT) 20VN, Random hexamers, or Gene Specific Primers can be selected. This reverse transcription premix allows for the generation of cDNA up to 12 kb in size within 15 minutes. RNA extracted from cells often contains genomic DNA contamination. If the genomic DNA is not removed before reverse transcription, both the genomic DNA and cDNA will be amplified during downstream qPCR reactions (especially when the primers are designed on the same exon), thus affecting the accuracy of gene expression quantification. This kit utilizes dsDNase to efficiently remove genomic DNA contamination. dsDNase can specifically digest double-stranded DNA (dsDNA or the DNA strand in DNA-RNA hybrid chains) and is thermally sensitive, rapidly and irreversibly inactivated at the reverse transcription temperature. Compared to the traditional method of using DNase I to remove genomic DNA contamination, dsDNase does not require the addition of EDTA for inactivation, saving experimental time and reducing inhibition of the reverse transcription reaction.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188436476279,"sku":"CLV-0038","price":93.13,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0038-0-cleavion-product.png?v=1781696346"},{"product_id":"m-mlv-giv-reverse-transcriptase","title":"M-MLV GIV Reverse Transcriptase","description":"M-MLV GIV Reverse Transcriptase is a fourth-generation M-MLV reverse transcriptase obtained through genetic modification and recombination technologies. Compared with the wild-type M-MLV reverse transcriptase, it lacks RNase H activity. Key enhancements include superior thermal stability, with an optimal reaction temperature of 55°C and sustained 50% activity at 70°C. Furthermore, it demonstrates improved tolerance to complex secondary structures , accelerated reaction speed (completing reactions in as little as 10 minutes), and high template affinity. These combined properties ensure robust performance with low-copy templates and enable efficient synthesis of full-length cDNA fragments up to 20 kb.","brand":"Cleavion","offers":[{"title":"10000 U","offer_id":57188436607351,"sku":"CLV-0039","price":93.13,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0039-0-cleavion-product.png?v=1781696346"},{"product_id":"taq-hs-sybr-green-qpcr-premix-no-rox","title":"Taq-HS SYBR Green qPCR Premix (No ROX)","description":"Taq-HS SYBR® Green qPCR Premix is a special premix for qPCR detection using SYBR® Green I chimeric fluorescence method. The Mix contains all components for qPCR, except DNA templates and primers. It reduces the reaction set-up time and risk of contaminations. The core component is the chemically modified hot-start Taq DNA polymerase. With the optimized buffer, the Mix suppresses non-specific amplifications well to obtain consistent and reliable qPCR results in a broad template concentration range.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188436771191,"sku":"CLV-0040","price":78.91,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0040-0-cleavion-product.png?v=1781696347"},{"product_id":"2x-taq-as-pcr-master-mix-with-loading-dye","title":"2x Taq-AS PCR Master Mix with Loading Dye","description":"The 2xTaq-AS PCR Mix (+Dye) includes Taq-AS DNA Polymerase, reaction buffer, dNTPs, and loading dye among other components. Taq-AS (Advanced Strong) DNA Polymerase is a screened variant capable of efficiently amplifying DNA fragments up to 5 kb. It exhibits excellent inhibitor tolerance and has a threefold faster amplification speed compared to regular Taq DNA polymerase. The PCR products do not require the addition of a loading buffer and can be directly loaded for gel electrophoresis. During the electrophoresis process, two indicator bands, one purple and one yellow, will appear.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188444569975,"sku":"CLV-0274","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0274-0-cleavion-product.png?v=1781696421"},{"product_id":"2x-taq-as-pcr-master-mix-with-loading-dye-1","title":"2x Taq-AS PCR Master Mix with Loading Dye","description":"The 2xTaq-AS PCR Mix (+Dye) includes Taq-AS DNA Polymerase, reaction buffer, dNTPs, and loading dye among other components. Taq-AS (Advanced Strong) DNA Polymerase is a screened variant capable of efficiently amplifying DNA fragments up to 5 kb. It exhibits excellent inhibitor tolerance and has a threefold faster amplification speed compared to regular Taq DNA polymerase. The PCR products do not require the addition of a loading buffer and can be directly loaded for gel electrophoresis. During the electrophoresis process, two indicator bands, one purple and one yellow, will appear.","brand":"Cleavion","offers":[{"title":"100x 1 mL","offer_id":57188444701047,"sku":"CLV-0275","price":997.7,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0275-0-cleavion-product.png?v=1781696422"},{"product_id":"2x-taq-as-pcr-master-mix","title":"2x Taq-AS PCR Master Mix","description":"The 2xTaq-AS PCR Mix includes Taq-AS DNA Polymerase, reaction buffer, dNTPs, and other components. Taq-AS (Advanced Strong) DNA Polymerase is a variant obtained through screening, capable of efficiently amplifying DNA fragments up to 5 kb. It exhibits excellent inhibitor tolerance and has a threefold faster amplification speed compared to regular Taq DNA polymerase.","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188444864887,"sku":"CLV-0276","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0276-0-cleavion-product.png?v=1781696425"},{"product_id":"phi29-dna-polymerase-for-rolling-circle-amplification","title":"Phi29 DNA Polymerase for Rolling Circle Amplification","description":"Phi29 II DNA Polymerase is engineered from the original phi29 DNA Polymerase with significantly improved performance. This enzyme is significantly improved over phi29 DNA polymerase in enhanced thermostability, amplification yield and sensitivity, while retaining all the benefits of the wild-type enzyme, with optimal amplification achieved at 42°C.","brand":"Cleavion","offers":[{"title":"500 U","offer_id":57188444963191,"sku":"CLV-0277","price":146.25,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0277-0-cleavion-product.png?v=1781696424"},{"product_id":"phi29-dna-polymerase-for-rolling-circle-amplification-1","title":"Phi29 DNA Polymerase for Rolling Circle Amplification","description":"Phi29 II DNA Polymerase is engineered from the original phi29 DNA Polymerase with significantly improved performance. This enzyme is significantly improved over phi29 DNA polymerase in enhanced thermostability, amplification yield and sensitivity, while retaining all the benefits of the wild-type enzyme, with optimal amplification achieved at 42°C.","brand":"Cleavion","offers":[{"title":"2500 U","offer_id":57188445127031,"sku":"CLV-0278","price":465.8,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0278-0-cleavion-product.png?v=1781696424"},{"product_id":"giv-all-in-one-master-mix-with-dsdnase","title":"GIV All-in-One Master Mix with dsDNase","description":"GIV All-in-One MasterMix (with dsDNase) is a simplified system specially designed for single-stranded cDNA synthesis. It contains all components required for the reaction, requiring only the addition of RNA template and water. The M-MLV GIV Reverse Transcriptase in the premix is a newly developed 4th-generation reverse transcriptase. It features faster reaction speed, completing the reverse transcription process within 5 minutes; stronger thermostability with an optimal reaction temperature of 55 °C; the cDNA product can reach up to 20 kb in length. Additionally, it exhibits tolerance to common inhibitors such as ethanol, isopropanol, humic acid, lithium chloride, and guanidine hydrochloride. dsDNase can specifically digest double-stranded DNA even in the presence of primers and probes, without degrading single-stranded DNA or RNA. It exhibits thermal sensitivity, enabling rapid irreversible inactivation at elevated temperatures to eliminate genomic contamination. This prevents dsDNase from damaging DNA within DNA-RNA hybrid strands during reverse transcription.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188445290871,"sku":"CLV-0279","price":360.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0279-0-cleavion-product.png?v=1781696426"},{"product_id":"2x-taq-plus-pcr-master-mix","title":"2x Taq-Plus PCR Master Mix","description":"\u003cstyle\u003e\n  .clv-seo-block {\n    --clv-navy: #06184a;\n    --clv-blue: #0b2f78;\n    --clv-cyan: #00c8f5;\n    --clv-cyan-soft: #e8fbff;\n    --clv-ice: #f6fcff;\n    --clv-text: #223047;\n    --clv-muted: #667085;\n    --clv-line: #d7edf5;\n    font-family: Arial, Helvetica, sans-serif;\n    color: var(--clv-text);\n    line-height: 1.65;\n    background: #ffffff;\n  }\n\n  .clv-seo-block * {\n    box-sizing: border-box;\n  }\n\n  .clv-seo-wrap {\n    max-width: 1180px;\n    margin: 0 auto;\n    padding: 28px 18px 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var(--clv-line);\n    border-left: 5px solid var(--clv-cyan);\n  }\n\n  .clv-cta h2 {\n    margin: 0 0 10px;\n    color: var(--clv-navy);\n  }\n\n  .clv-cta p {\n    margin: 0;\n  }\n\n  @media (max-width: 900px) {\n    .clv-grid,\n    .clv-two-col,\n    .clv-workflow {\n      grid-template-columns: 1fr;\n    }\n\n    .clv-hero,\n    .clv-section,\n    .clv-highlight,\n    .clv-cta {\n      padding: 22px;\n    }\n  }\n\u003c\/style\u003e\n\u003cdiv class=\"clv-seo-block\"\u003e\n\u003cdiv class=\"clv-seo-wrap\"\u003e\n\u003csection class=\"clv-hero\"\u003e\u003cspan class=\"clv-eyebrow\"\u003ePCR Polymerase Master Mix\u003c\/span\u003e\n\u003ch2\u003e2x Taq-Plus PCR Master Mix for Routine PCR Amplification\u003c\/h2\u003e\n\u003cp\u003eCleavion 2x Taq-Plus PCR Master Mix is a ready-to-use PCR premix designed for convenient, consistent DNA amplification. The 2x formulation combines Taq DNA Polymerase, reaction buffer, dNTPs, and optimized PCR components in one tube, reducing preparation time and helping improve repeatability across routine molecular biology workflows.\u003c\/p\u003e\n\u003cdiv class=\"clv-badges\"\u003e\n\u003cspan class=\"clv-badge\"\u003eSKU: CLV-0305\u003c\/span\u003e \u003cspan class=\"clv-badge\"\u003eFormat: 5 × 1 mL\u003c\/span\u003e \u003cspan class=\"clv-badge\"\u003eStorage: -20 °C\u003c\/span\u003e \u003cspan class=\"clv-badge\"\u003eFor PCR products up to 5 kb\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"clv-grid\"\u003e\n\u003carticle class=\"clv-card\"\u003e\n\u003ch3\u003eReady-to-use 2x PCR premix\u003c\/h3\u003e\n\u003cp\u003eThe master mix format simplifies reaction setup by reducing the number of individual reagents that must be pipetted into each PCR tube.\u003c\/p\u003e\n\u003c\/article\u003e\n\u003carticle class=\"clv-card\"\u003e\n\u003ch3\u003eOptimized for routine PCR\u003c\/h3\u003e\n\u003cp\u003eSuitable for standard endpoint PCR, colony screening, template verification, insert checking, and general DNA amplification tasks.\u003c\/p\u003e\n\u003c\/article\u003e\n\u003carticle class=\"clv-card\"\u003e\n\u003ch3\u003eDirect T\/A cloning compatibility\u003c\/h3\u003e\n\u003cp\u003ePCR products generated with Taq-based amplification can be used for downstream T\/A cloning workflows when compatible with the selected cloning system.\u003c\/p\u003e\n\u003c\/article\u003e\n\u003c\/div\u003e\n\u003csection class=\"clv-section\"\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e2x Taq-Plus PCR Master Mix is formulated for researchers who need reliable PCR amplification with fewer preparation steps. Because the mix already contains polymerase, buffer, dNTPs, and supporting reaction components, users only need to add template DNA, primers, and nuclease-free water to prepare the final PCR reaction.\u003c\/p\u003e\n\u003cp\u003eThis PCR master mix is especially useful for laboratories running repeated PCR assays, screening multiple samples, or standardizing reaction setup between users. The premixed format helps reduce pipetting variation and supports efficient setup of single reactions or larger sample batches.\u003c\/p\u003e\n\u003cdiv class=\"clv-two-col\"\u003e\n\u003cdiv class=\"clv-card\"\u003e\n\u003ch3\u003eRecommended Applications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine PCR amplification\u003c\/li\u003e\n\u003cli\u003eDNA fragment amplification up to 5 kb\u003c\/li\u003e\n\u003cli\u003eColony PCR and clone screening\u003c\/li\u003e\n\u003cli\u003eGenotyping PCR workflows\u003c\/li\u003e\n\u003cli\u003eTemplate confirmation and insert verification\u003c\/li\u003e\n\u003cli\u003ePreparation of PCR products for T\/A cloning\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-card\"\u003e\n\u003ch3\u003eKey Advantages\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eConvenient 2x master mix format\u003c\/li\u003e\n\u003cli\u003eReduced pipetting steps during PCR setup\u003c\/li\u003e\n\u003cli\u003eConsistent reagent composition between reactions\u003c\/li\u003e\n\u003cli\u003eGood amplification specificity for routine targets\u003c\/li\u003e\n\u003cli\u003eCompatible with common molecular biology workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-section\"\u003e\n\u003ch2\u003eTechnical Specifications\u003c\/h2\u003e\n\u003ctable class=\"clv-spec-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Name\u003c\/th\u003e\n\u003ctd\u003e2x Taq-Plus PCR Master Mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog \/ SKU\u003c\/th\u003e\n\u003ctd\u003eCLV-0305\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Type\u003c\/th\u003e\n\u003ctd\u003ePCR master mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCategory\u003c\/th\u003e\n\u003ctd\u003ePolymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSubcategory\u003c\/th\u003e\n\u003ctd\u003eConventional DNA polymerase \u0026amp; PCR mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEnzyme System\u003c\/th\u003e\n\u003ctd\u003eTaq DNA Polymerase-based PCR premix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003e2x ready-to-use liquid master mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003ctd\u003e5 × 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAmplification Range\u003c\/th\u003e\n\u003ctd\u003eDesigned for PCR products up to 5 kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage Conditions\u003c\/th\u003e\n\u003ctd\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-highlight\"\u003e\n\u003ch2\u003eHow to Use 2x Taq-Plus PCR Master Mix\u003c\/h2\u003e\n\u003cp\u003eThe 2x concentration is intended to be diluted to 1x in the final PCR reaction. In a typical workflow, combine the master mix with forward primer, reverse primer, DNA template, and nuclease-free water according to the final reaction volume required by your assay.\u003c\/p\u003e\n\u003cdiv class=\"clv-workflow\"\u003e\n\u003cdiv class=\"clv-step\"\u003e\n\u003cstrong\u003e1. Thaw \u0026amp; mix\u003c\/strong\u003e Bring the master mix to working temperature and mix gently before use.\u003c\/div\u003e\n\u003cdiv class=\"clv-step\"\u003e\n\u003cstrong\u003e2. Add primers\u003c\/strong\u003e Add forward and reverse primers according to your validated PCR design.\u003c\/div\u003e\n\u003cdiv class=\"clv-step\"\u003e\n\u003cstrong\u003e3. Add template\u003c\/strong\u003e Add DNA template and nuclease-free water to reach the final reaction volume.\u003c\/div\u003e\n\u003cdiv class=\"clv-step\"\u003e\n\u003cstrong\u003e4. Run PCR\u003c\/strong\u003e Use cycling conditions suitable for the template, primer pair, and expected amplicon length.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-section\"\u003e\n\u003ch2\u003eWhy Choose Cleavion 2x Taq-Plus PCR Master Mix?\u003c\/h2\u003e\n\u003cp\u003eCleavion 2x Taq-Plus PCR Master Mix is designed for laboratories that need a practical PCR reagent for everyday amplification tasks. The premixed format supports efficient preparation, helps reduce manual reagent handling, and allows researchers to focus on template quality, primer design, and PCR optimization.\u003c\/p\u003e\n\u003cp\u003eFor workflows such as clone screening, routine gene amplification, sample verification, and T\/A cloning preparation, this Taq-based PCR master mix offers a convenient balance of usability and performance.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-faq\"\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cdiv class=\"clv-faq-grid\"\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat is 2x Taq-Plus PCR Master Mix used for?\u003c\/h3\u003e\n\u003cp\u003eIt is used for routine PCR amplification of DNA fragments. Common applications include endpoint PCR, colony PCR, clone screening, genotyping workflows, and insert verification.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat does “2x” mean in a PCR master mix?\u003c\/h3\u003e\n\u003cp\u003e“2x” means the master mix is supplied at twice the final working concentration. It is normally combined with primers, template DNA, and water so that the final PCR reaction contains the master mix at 1x concentration.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat components are included in the master mix?\u003c\/h3\u003e\n\u003cp\u003eThe mix contains Taq DNA Polymerase, PCR reaction buffer, dNTPs, and optimized components required for standard PCR setup. Users add primers, DNA template, and nuclease-free water.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eCan PCR products from this mix be used for T\/A cloning?\u003c\/h3\u003e\n\u003cp\u003eYes. PCR products generated with this Taq-based master mix can be directly used for T\/A cloning workflows when the downstream cloning system is compatible.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat is the recommended storage temperature?\u003c\/h3\u003e\n\u003cp\u003eStore Cleavion 2x Taq-Plus PCR Master Mix at -20 °C. Avoid unnecessary freeze-thaw cycles and mix gently before use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"clv-faq-item\"\u003e\n\u003ch3\u003eWhat amplicon size can this master mix amplify?\u003c\/h3\u003e\n\u003cp\u003eThe product is designed to amplify DNA fragments up to 5 kb, depending on template quality, primer design, GC content, and PCR cycling conditions.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"clv-cta\"\u003e\n\u003ch2\u003eCleavion PCR Reagents for Molecular Biology Workflows\u003c\/h2\u003e\n\u003cp\u003eUse 2x Taq-Plus PCR Master Mix when you need a convenient, Taq-based PCR premix for routine amplification, screening, and cloning-related workflows. Its ready-to-use 2x format helps simplify setup while supporting consistent PCR performance across repeated assays.\u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\",\n  \"@type\": \"Product\",\n  \"name\": \"2x Taq-Plus PCR Master Mix\",\n  \"sku\": \"CLV-0305\",\n  \"brand\": {\n    \"@type\": \"Brand\",\n    \"name\": \"Cleavion\"\n  },\n  \"category\": \"PCR Master Mix\",\n  \"description\": \"Cleavion 2x Taq-Plus PCR Master Mix is a ready-to-use Taq DNA Polymerase-based PCR premix containing reaction buffer, dNTPs, and optimized components for routine PCR amplification up to 5 kb.\",\n  \"url\": \"https:\/\/cleavion.com\/products\/2x-taq-plus-pcr-master-mix\",\n  \"additionalProperty\": [\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Catalog number\",\n      \"value\": \"CLV-0305\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Format\",\n      \"value\": \"2x ready-to-use PCR master mix\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Pack size\",\n      \"value\": \"5 × 1 mL\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Storage conditions\",\n      \"value\": \"-20 °C\"\n    },\n    {\n      \"@type\": \"PropertyValue\",\n      \"name\": \"Application\",\n      \"value\": \"Routine PCR amplification, colony PCR, clone screening, genotyping, T\/A cloning preparation\"\n    }\n  ]\n}\n\u003c\/script\u003e\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Cleavion","offers":[{"title":"5x 1 mL","offer_id":57188446011767,"sku":"CLV-0305","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0305-0-cleavion-product.png?v=1781696440"}],"url":"https:\/\/cleavion.com\/collections\/pcr-qpcr-reverse-transcription.oembed?page=2","provider":"Cleavion Inc.","version":"1.0","type":"link"}