{"title":"Restriction \u0026 DNA Modification","description":"\u003cp\u003eExplore our extensive range of restriction and DNA modification enzymes, including Type II restriction enzymes, rapid-digest variants, nicking and homing endonucleases, ligases, recombinases, and nucleases. Engineered for precision and reliability, these enzymes support a broad spectrum of molecular biology applications from routine DNA digestion and ligation to advanced genome manipulation and nucleic acid processing.\u003c\/p\u003e\u003cp\u003eEvery enzyme in this collection is rigorously quality-controlled for activity, specificity, and freedom from contaminating nucleases, ensuring consistent and reproducible results across experiments. Whether you're performing standard cloning, site-specific nicking, or RNA processing, our portfolio provides the tools researchers need to work with confidence at every stage of their workflow.\u003c\/p\u003e","products":[{"product_id":"t4-uvsx-recombinase","title":"T4 UvsX Recombinase","description":"T4 UvsX Recombinase, derived from T4 bacteriophage, is a homolog of the RecA\/Rad51 recombinase family with a molecular weight of 45 kDa. The RecA\/Rad51 recombinase family plays a crucial role in the repair of double-stranded DNA breaks and the restart of replication forks. T4 UvsX Recombinase can bind to single-stranded DNA (primer) to form a complex, which then searches for homologous sequences within double-stranded DNA and catalyzes strand displacement reactions. In combination with other related proteins, UvsY can facilitate isothermal amplification reactions.","brand":"Cleavion","offers":[{"title":"500 µg","offer_id":57188435362167,"sku":"CLV-0008","price":239.41,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0008-0-cleavion-product.png?v=1781696338"},{"product_id":"et-ssb","title":"ET SSB","description":"ET SSB (Extreme Thermostable single-strand DNA Binding Protein) is a single-stranded DNA binding protein isolated from thermophilic microorganisms, with a molecular weight of 16 kDa. It retains full activity even after incubation at 95°Cfor 60 minutes. Due to its extremely high thermal stability, ET SSB can be used in applications that require high-temperature conditions, such as nucleic acid amplification and sequencing.","brand":"Cleavion","offers":[{"title":"100 µg","offer_id":57188436640119,"sku":"CLV-0016","price":79.04,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0016-0-cleavion-product.png?v=1781696345"},{"product_id":"nt-bstnbi-restriction-enzyme","title":"Nt.BstNBI Restriction Enzyme","description":"","brand":"Cleavion","offers":[{"title":"2000 U","offer_id":57188441784695,"sku":"CLV-0158","price":148.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0158-0-cleavion-product.png?v=1781696388"},{"product_id":"pspgi-ecorii-restriction-enzyme","title":"PspGI (EcoRII) Restriction Enzyme","description":"\u003cp\u003e\u003cstrong\u003eRapid-digestion restriction enzymes\u003c\/strong\u003e are engineered restriction enzymes designed for fast DNA digestion. These enzymes show strong activity in a \u003cstrong\u003euniversal digestion buffer\u003c\/strong\u003e and can digest DNA within \u003cstrong\u003e5–15 minutes\u003c\/strong\u003e. This enables different combinations of restriction enzymes to work simultaneously in a single reaction tube and reduces the need for sequential digestion steps.\u003c\/p\u003e\n\u003cp\u003eRapid-digestion restriction enzymes undergo strict quality control testing and can be used to digest \u003cstrong\u003eplasmid DNA, genomic DNA, viral DNA, and PCR products\u003c\/strong\u003e. The \u003cstrong\u003ecolor loading digestion buffer\u003c\/strong\u003e contains a density reagent together with red and yellow tracking dyes, allowing reaction mixtures to be loaded directly onto an agarose gel after digestion. In a \u003cstrong\u003e1% agarose gel\u003c\/strong\u003e, the red dye migrates similarly to approximately \u003cstrong\u003e2.5 kb double-stranded DNA fragments\u003c\/strong\u003e, while the yellow dye migrates similarly to approximately \u003cstrong\u003e10 bp double-stranded DNA fragments\u003c\/strong\u003e.\u003c\/p\u003e","brand":"Cleavion","offers":[{"title":"200 Reactions - 2000 U","offer_id":57188441915767,"sku":"CLV-0115","price":65.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0115-0-cleavion-product.png?v=1781696390"},{"product_id":"nb-bbvci-restriction-enzyme","title":"Nb.BbvCI Restriction Enzyme","description":"","brand":"Cleavion","offers":[{"title":"300 U","offer_id":57188441948535,"sku":"CLV-0159","price":65.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0159-0-cleavion-product.png?v=1781696388"},{"product_id":"t4-rna-ligase-1","title":"T4 RNA Ligase 1","description":"T4 RNA Ligase 1 is an ATP-dependent ligase derived from bacteriophage T4. It catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3 hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond with hydrolysis of ATP to AMP and PPi. The ligation efficiency of T4 RNA Ligase 1 varies significantly for different substrates, following the order: ssRNA-ssRNA\u0026gt;ssRNA-ssDNA\u0026gt;ssDNA-ssDNA. Additionally, T4 RNA Ligase 1 can also be used for ligation between RNA and mononucleotides, provided that the mononucleotides are phosphorylated at both the 5' and 3' ends. This application is commonly used for 3'-end labeling of RNA.","brand":"Cleavion","offers":[{"title":"1000 U","offer_id":57188441981303,"sku":"CLV-0197","price":96.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0197-0-cleavion-product.png?v=1781696389"},{"product_id":"nb-bsrdi-restriction-enzyme","title":"Nb.BsrDI Restriction Enzyme","description":"","brand":"Cleavion","offers":[{"title":"2000 U","offer_id":57188442079607,"sku":"CLV-0160","price":148.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0160-0-cleavion-product.png?v=1781696389"},{"product_id":"t4-rna-ligase-2","title":"T4 RNA Ligase 1","description":"T4 RNA Ligase 1 is an ATP-dependent ligase derived from bacteriophage T4. It catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3 hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond with hydrolysis of ATP to AMP and PPi. The ligation efficiency of T4 RNA Ligase 1 varies significantly for different substrates, following the order: ssRNA-ssRNA\u0026gt;ssRNA-ssDNA\u0026gt;ssDNA-ssDNA. Additionally, T4 RNA Ligase 1 can also be used for ligation between RNA and mononucleotides, provided that the mononucleotides are phosphorylated at both the 5' and 3' ends. This application is commonly used for 3'-end labeling of RNA.","brand":"Cleavion","offers":[{"title":"5000 U","offer_id":57188442145143,"sku":"CLV-0198","price":266.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0198-0-cleavion-product.png?v=1781696389"},{"product_id":"assembly-kit-bsmbi-t4-dna-ligase","title":"Assembly Kit (BsmBI+T4 DNA ligase)","description":"Golden Gate Assembly Kit share the same single-step precision cloning strategy, popularly known as \"Golden Gate Assembly\", that relies on the unique properties of type IIs restriction enzymes to generate compatible ends on DNA fragments that are then joined together by the T4 DNA ligase. The Kit are particularly adept at assembling difficult to-clone sequences such as repetitive and very small sequences (\u0026lt;100 bp), gene variants, and TAL (transcription activator-like) effector genes. Type IIS restriction enzymes bind to their recognition sites but cut the DNA downstream from that site at a positional, not sequence specific, cut site. Thus, a single Type IIS restriction enzyme can be used to generate DNA fragments with unique overhangs. The cloning procedure is as follows: Design recognition sites for type IIS restriction enzymes outside the cleavage sites of the target gene. Assembly of digested fragments proceeds through annealing of complementary four base overhangs on adjacent fragments. The digested fragments and the final assembly no longer contain Type IIS restriction enzyme recognition sites, so no further cutting is possible. The vector contains sticky ends complementary to those of the target gene, enabling ligation without introducing extraneous sequences, thereby achieving seamless cloning. The Golden Gate Assembly Kit (BsmBI), based on the above principle, contains all enzymes required for restriction digestion and ligation in a ready-to-use Mix for convenient pipetting. It supports ligation of up to 16 fragments in a single reaction, fully satisfying diverse experimental requirements.","brand":"Cleavion","offers":[{"title":"10 Reactions","offer_id":57188442243447,"sku":"CLV-0199","price":77.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0199-0-cleavion-product.png?v=1781696390"},{"product_id":"assembly-kit-bsmbi-t4-dna-ligase-1","title":"Assembly Kit (BsmBI+T4 DNA ligase)","description":"Golden Gate Assembly Kit share the same single-step precision cloning strategy, popularly known as \"Golden Gate Assembly\", that relies on the unique properties of type IIs restriction enzymes to generate compatible ends on DNA fragments that are then joined together by the T4 DNA ligase. 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The vector contains sticky ends complementary to those of the target gene, enabling ligation without introducing extraneous sequences, thereby achieving seamless cloning. The Golden Gate Assembly Kit (BsmBI), based on the above principle, contains all enzymes required for restriction digestion and ligation in a ready-to-use Mix for convenient pipetting. It supports ligation of up to 16 fragments in a single reaction, fully satisfying diverse experimental requirements.","brand":"Cleavion","offers":[{"title":"50 Reactions","offer_id":57188442341751,"sku":"CLV-0200","price":132.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0200-0-cleavion-product.png?v=1781696391"},{"product_id":"bpii-bbsi-type-iis-restriction-enzyme","title":"BpiI\/BbsI Type IIS Restriction Enzyme","description":"\u003cp\u003eBpiI is a Type IIS restriction enzyme. 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It is one of the commonly used enzymes for Golden Gate assembly and is also suitable for routine cloning, restriction digestion, and DNA fragment identification.\u003c\/p\u003e","brand":"Cleavion","offers":[{"title":"250 U","offer_id":57188443062647,"sku":"CLV-0284","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0284-0-cleavion-product.png?v=1781696416"},{"product_id":"dnase-i-st-rnase-free","title":"DNase I-ST, RNase-Free","description":"This product is an engineered variant of DNase I, which exhibits significantly improved salt tolerance compared to the wild-type enzyme. It can fully digest DNA in 300 mM salt. This product is free from RNase contamination.","brand":"Cleavion","offers":[{"title":"1000 U","offer_id":57188443357559,"sku":"CLV-0266","price":103.39,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0266-0-cleavion-product.png?v=1781696418"},{"product_id":"apeki-restriction-enzyme-for-precision-dna-digestion","title":"ApeKI Restriction Enzyme for Precision DNA Digestion","description":"ApeKI is a Type IIP restriction enzyme derived from the Aeropyrum pernix K1. It recognizes and cleaves the sequence G\/CWGC, generating sticky-end with a 3-base overhang. ApeKI is one of the preferred enzymes for Genotyping-by-Sequencing (GBS) and is widely used in plant and animal genetics, population evolution studies, and functional gene mapping.","brand":"Cleavion","offers":[{"title":"500 U","offer_id":57188444406135,"sku":"CLV-0273","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0273-0-cleavion-product.png?v=1781696421"},{"product_id":"bspei-restriction-enzyme-for-precision-dna-digestion","title":"BspEI Restriction Enzyme for Precision DNA Digestion","description":"BspEI is a Type IIP restriction enzyme derived from Bacillus and obtained by recombinant expression and purification in Escherichia coli. BspEI recognizes and cleaves the sequence TCCGGA, generating a 4-base 5' overhang. 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It features faster reaction speed, completing the reverse transcription process within 5 minutes; stronger thermostability with an optimal reaction temperature of 55 °C; the cDNA product can reach up to 20 kb in length. Additionally, it exhibits tolerance to common inhibitors such as ethanol, isopropanol, humic acid, lithium chloride, and guanidine hydrochloride. dsDNase can specifically digest double-stranded DNA even in the presence of primers and probes, without degrading single-stranded DNA or RNA. It exhibits thermal sensitivity, enabling rapid irreversible inactivation at elevated temperatures to eliminate genomic contamination. This prevents dsDNase from damaging DNA within DNA-RNA hybrid strands during reverse transcription.","brand":"Cleavion","offers":[{"title":"100 Reactions","offer_id":57188445290871,"sku":"CLV-0279","price":360.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0279-0-cleavion-product.png?v=1781696426"},{"product_id":"swai-restriction-enzyme-for-precision-dna-digestion","title":"SwaI Restriction Enzyme for Precision DNA Digestion","description":"","brand":"Cleavion","offers":[{"title":"1000 U","offer_id":57188445684087,"sku":"CLV-0300","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0300-0-cleavion-product.png?v=1781696426"},{"product_id":"esp3i-bsmbi-type-iis-restriction-enzyme-adcf","title":"Esp3I\/BsmBI Type IIS Restriction Enzyme, ADCF","description":"","brand":"Cleavion","offers":[{"title":"2000 U","offer_id":57188445880695,"sku":"CLV-0301","price":758.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0301-0-cleavion-product.png?v=1781696438"},{"product_id":"ecori-restriction-enzyme-adcf","title":"EcoRI Restriction Enzyme, ADCF","description":"","brand":"Cleavion","offers":[{"title":"5000 U","offer_id":57188445913463,"sku":"CLV-0302","price":226.85,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0302-0-cleavion-product.png?v=1781696439"},{"product_id":"rnase-gg-for-rna-digestion","title":"RNase GG for RNA Digestion","description":"","brand":"Cleavion","offers":[{"title":"2500 U","offer_id":57188445946231,"sku":"CLV-0303","price":218.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0303-0-cleavion-product.png?v=1781696439"},{"product_id":"i-ceui-homing-endonuclease","title":"I-CeuI Homing Endonuclease","description":"","brand":"Cleavion","offers":[{"title":"250 U","offer_id":57188445978999,"sku":"CLV-0304","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0304-0-cleavion-product.png?v=1781696440"}],"url":"https:\/\/cleavion.com\/collections\/restriction-dna-modification.oembed","provider":"Cleavion Inc.","version":"1.0","type":"link"}