{"title":"Restriction Enzymes","description":"\u003cp\u003eCleavion Standard \u0026amp; Specialty Restriction Enzymes covers restriction enzymes supplied as standalone products outside the rapid enzyme series, including specialty enzymes, rare cutters and commonly used cloning enzymes. This collection keeps individual enzyme products separate from kits and assay reagents.\u003c\/p\u003e\u003cp\u003eUse this page for customers searching by enzyme name or catalog number. The recommended Shopify setup is to apply a clean restriction-enzyme tag during import, then create filters for enzyme name, units and grade so researchers can quickly find the exact product they need.\u003c\/p\u003e","products":[{"product_id":"esp3i-bsmbi-restriction-enzyme","title":"Esp3I (BsmBI) Restriction Enzyme","description":"LightNing® enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All LightNing® enzymes show superior activity in the universal CutOne® and CutOne® Color Buffer, and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. LightNing® enzymes have passed multiple strict quality controls, and can be used to digest plasmid, genomic and viral DNA as well as PCR products. CutOne® Color Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the CutOne® Color Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.","brand":"Cleavion","offers":[{"title":"30 Reactions - 300 U","offer_id":57188435263863,"sku":"CLV-0088","price":65.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0088-0-cleavion-product.png?v=1781696338"},{"product_id":"bsmbi-restriction-enzyme","title":"BsmBI Restriction Enzyme","description":"BsmBI is a Type IIS restriction enzyme that recognizes non-palindromic sequences and cuts outside the recognition site. It is commonly used in Golden Gate assembly. The reaction buffer has been optimized to maximize the functionality of BsmBI. Additionally, the reaction buffer contains recombinant albumin, which enhances the stability of various enzymes.","brand":"Cleavion","offers":[{"title":"200 U","offer_id":57188437426551,"sku":"CLV-0141","price":65.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0141-0-cleavion-product.png?v=1781696375"},{"product_id":"bsmbi-restriction-enzyme-1","title":"BsmBI Restriction Enzyme","description":"BsmBI is a Type IIS restriction enzyme that recognizes non-palindromic sequences and cuts outside the recognition site. It is commonly used in Golden Gate assembly. The reaction buffer has been optimized to maximize the functionality of BsmBI. Additionally, the reaction buffer contains recombinant albumin, which enhances the stability of various enzymes.","brand":"Cleavion","offers":[{"title":"1000 U","offer_id":57188437524855,"sku":"CLV-0142","price":134.38,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0142-0-cleavion-product.png?v=1781696377"},{"product_id":"bspqi-restriction-enzyme","title":"BspQI Restriction Enzyme","description":"","brand":"Cleavion","offers":[{"title":"500 U","offer_id":57188437754231,"sku":"CLV-0143","price":65.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0143-0-cleavion-product.png?v=1781696377"},{"product_id":"bsrdi-restriction-enzyme","title":"BsrDI Restriction Enzyme","description":"","brand":"Cleavion","offers":[{"title":"250 U","offer_id":57188437983607,"sku":"CLV-0144","price":65.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0144-0-cleavion-product.png?v=1781696378"},{"product_id":"bstxi-restriction-enzyme","title":"BstXI Restriction Enzyme","description":"","brand":"Cleavion","offers":[{"title":"500 U","offer_id":57188438180215,"sku":"CLV-0145","price":65.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0145-0-cleavion-product.png?v=1781696379"},{"product_id":"pcii-restriction-enzyme","title":"PciI Restriction Enzyme","description":"","brand":"Cleavion","offers":[{"title":"200 U","offer_id":57188438442359,"sku":"CLV-0146","price":65.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0146-0-cleavion-product.png?v=1781696380"},{"product_id":"sgrai-restriction-enzyme","title":"SgrAI Restriction Enzyme","description":"","brand":"Cleavion","offers":[{"title":"500 U","offer_id":57188440113527,"sku":"CLV-0148","price":65.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0148-0-cleavion-product.png?v=1781696381"},{"product_id":"sspdi-kasi-restriction-enzyme","title":"SspDI (KasI) Restriction Enzyme","description":"","brand":"Cleavion","offers":[{"title":"250 U","offer_id":57188440277367,"sku":"CLV-0149","price":65.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0149-0-cleavion-product.png?v=1781696382"},{"product_id":"xmni-restriction-enzyme","title":"XmnI Restriction Enzyme","description":"","brand":"Cleavion","offers":[{"title":"500 U","offer_id":57188440441207,"sku":"CLV-0150","price":65.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0150-0-cleavion-product.png?v=1781696382"},{"product_id":"bspqi-restriction-enzyme-gmp-grade","title":"BspQI Restriction Enzyme, GMP Grade","description":"","brand":"Cleavion","offers":[{"title":"10000 U","offer_id":57188440736119,"sku":"CLV-0152","price":617.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0152-0-cleavion-product.png?v=1781696384"},{"product_id":"nt-bbvci-restriction-enzyme","title":"Nt.BbvCI Restriction Enzyme","description":"","brand":"Cleavion","offers":[{"title":"150 U","offer_id":57188441293175,"sku":"CLV-0156","price":65.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0156-0-cleavion-product.png?v=1781696387"},{"product_id":"nt-bspqi-restriction-enzyme","title":"Nt.BspQI Restriction Enzyme","description":"","brand":"Cleavion","offers":[{"title":"2000 U","offer_id":57188441653623,"sku":"CLV-0157","price":148.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0157-0-cleavion-product.png?v=1781696387"},{"product_id":"nb-bbvci-restriction-enzyme","title":"Nb.BbvCI Restriction Enzyme","description":"","brand":"Cleavion","offers":[{"title":"300 U","offer_id":57188441948535,"sku":"CLV-0159","price":65.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0159-0-cleavion-product.png?v=1781696388"},{"product_id":"bbvci-restriction-enzyme","title":"BbvCI Restriction Enzyme","description":"\u003cp\u003e\u003cstrong\u003eBbvCI\u003c\/strong\u003e is derived from the \u003cem\u003eBbvCI\u003c\/em\u003e gene of \u003cem\u003eBacillus brevis\u003c\/em\u003e and is obtained through recombinant expression in \u003cem\u003eEscherichia coli\u003c\/em\u003e. BbvCI is a Type IIA restriction enzyme that exists as a heterodimer composed of two distinct catalytic subunits, each with an independent cleavage site.\u003c\/p\u003e\n\u003cp\u003eIt recognizes and cleaves the non-palindromic sequence \u003cstrong\u003eCCTCAGC (-5\/-2)\u003c\/strong\u003e, generating \u003cstrong\u003e5′ cohesive ends with 3-base overhangs\u003c\/strong\u003e. BbvCI uses the \u003cstrong\u003erecommended reaction buffer\u003c\/strong\u003e and is compatible with double digestion with other \u003cstrong\u003erapid-digestion restriction enzymes\u003c\/strong\u003e. However, extended digestion exceeding \u003cstrong\u003e3 hours\u003c\/strong\u003e should be avoided to reduce the risk of star activity.\u003c\/p\u003e","brand":"Cleavion","offers":[{"title":"50 U","offer_id":57188442046839,"sku":"CLV-0139","price":65.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0139-0-cleavion-product.png?v=1781696389"},{"product_id":"nb-bsrdi-restriction-enzyme","title":"Nb.BsrDI Restriction Enzyme","description":"","brand":"Cleavion","offers":[{"title":"2000 U","offer_id":57188442079607,"sku":"CLV-0160","price":148.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0160-0-cleavion-product.png?v=1781696389"},{"product_id":"bsiwi-restriction-enzyme","title":"BsiWI Restriction Enzyme","description":"BsiWI is a Type IIP restriction enzyme that recognizes palindromic sequences. The reaction buffer has been optimized to maximize the functionality of BsiWI. Additionally, the reaction buffer contains recombinant albumin, which enhances the stability of various enzymes.","brand":"Cleavion","offers":[{"title":"300 U","offer_id":57188442210679,"sku":"CLV-0140","price":65.53,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0140-0-cleavion-product.png?v=1781696390"},{"product_id":"assembly-kit-bsmbi-t4-dna-ligase","title":"Assembly Kit (BsmBI+T4 DNA ligase)","description":"Golden Gate Assembly Kit share the same single-step precision cloning strategy, popularly known as \"Golden Gate Assembly\", that relies on the unique properties of type IIs restriction enzymes to generate compatible ends on DNA fragments that are then joined together by the T4 DNA ligase. The Kit are particularly adept at assembling difficult to-clone sequences such as repetitive and very small sequences (\u0026lt;100 bp), gene variants, and TAL (transcription activator-like) effector genes. Type IIS restriction enzymes bind to their recognition sites but cut the DNA downstream from that site at a positional, not sequence specific, cut site. Thus, a single Type IIS restriction enzyme can be used to generate DNA fragments with unique overhangs. The cloning procedure is as follows: Design recognition sites for type IIS restriction enzymes outside the cleavage sites of the target gene. Assembly of digested fragments proceeds through annealing of complementary four base overhangs on adjacent fragments. The digested fragments and the final assembly no longer contain Type IIS restriction enzyme recognition sites, so no further cutting is possible. The vector contains sticky ends complementary to those of the target gene, enabling ligation without introducing extraneous sequences, thereby achieving seamless cloning. The Golden Gate Assembly Kit (BsmBI), based on the above principle, contains all enzymes required for restriction digestion and ligation in a ready-to-use Mix for convenient pipetting. It supports ligation of up to 16 fragments in a single reaction, fully satisfying diverse experimental requirements.","brand":"Cleavion","offers":[{"title":"10 Reactions","offer_id":57188442243447,"sku":"CLV-0199","price":77.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0199-0-cleavion-product.png?v=1781696390"},{"product_id":"assembly-kit-bsmbi-t4-dna-ligase-1","title":"Assembly Kit (BsmBI+T4 DNA ligase)","description":"Golden Gate Assembly Kit share the same single-step precision cloning strategy, popularly known as \"Golden Gate Assembly\", that relies on the unique properties of type IIs restriction enzymes to generate compatible ends on DNA fragments that are then joined together by the T4 DNA ligase. The Kit are particularly adept at assembling difficult to-clone sequences such as repetitive and very small sequences (\u0026lt;100 bp), gene variants, and TAL (transcription activator-like) effector genes. Type IIS restriction enzymes bind to their recognition sites but cut the DNA downstream from that site at a positional, not sequence specific, cut site. Thus, a single Type IIS restriction enzyme can be used to generate DNA fragments with unique overhangs. The cloning procedure is as follows: Design recognition sites for type IIS restriction enzymes outside the cleavage sites of the target gene. Assembly of digested fragments proceeds through annealing of complementary four base overhangs on adjacent fragments. The digested fragments and the final assembly no longer contain Type IIS restriction enzyme recognition sites, so no further cutting is possible. The vector contains sticky ends complementary to those of the target gene, enabling ligation without introducing extraneous sequences, thereby achieving seamless cloning. The Golden Gate Assembly Kit (BsmBI), based on the above principle, contains all enzymes required for restriction digestion and ligation in a ready-to-use Mix for convenient pipetting. It supports ligation of up to 16 fragments in a single reaction, fully satisfying diverse experimental requirements.","brand":"Cleavion","offers":[{"title":"50 Reactions","offer_id":57188442341751,"sku":"CLV-0200","price":132.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0200-0-cleavion-product.png?v=1781696391"},{"product_id":"bsmbi-elisa-kit","title":"BsmBI ELISA Kit","description":"This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for BsmBI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BsmBI present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for BsmBI is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BsmBI bound in the initial step. The color development is stopped and the intensity of the color is measured. And the residual degree of BsmBI in the sample is measured. Detection range: 0.156~10 ng\/ml Quantification limit: 0.156 ng\/ml Detection limit: 0.07 ng\/ml","brand":"Cleavion","offers":[{"title":"96 Tests","offer_id":57188443783543,"sku":"CLV-0269","price":466.69,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0269-0-cleavion-product.png?v=1781696420"},{"product_id":"aari-restriction-enzyme-for-precision-dna-digestion","title":"AarI Restriction Enzyme for Precision DNA Digestion","description":"AarI is an isoschizomer of PaqCI. Type IIS restriction enzyme which recognizes the sequence CACCTGC and cleaves outside of their recognition sequence, generating 4-base 5' overhangs. Especially, AarI requires two or more sites for digestion. AarI Activator supplied to improve cleavage. It recognizes a 7-basesequence that occurs infrequently in genes, is particularly suitable for Golden Gate assembly.","brand":"Cleavion","offers":[{"title":"100 U","offer_id":57188444242295,"sku":"CLV-0272","price":155.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0272-0-cleavion-product.png?v=1781696421"},{"product_id":"apeki-restriction-enzyme-for-precision-dna-digestion","title":"ApeKI Restriction Enzyme for Precision DNA Digestion","description":"ApeKI is a Type IIP restriction enzyme derived from the Aeropyrum pernix K1. It recognizes and cleaves the sequence G\/CWGC, generating sticky-end with a 3-base overhang. ApeKI is one of the preferred enzymes for Genotyping-by-Sequencing (GBS) and is widely used in plant and animal genetics, population evolution studies, and functional gene mapping.","brand":"Cleavion","offers":[{"title":"500 U","offer_id":57188444406135,"sku":"CLV-0273","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0273-0-cleavion-product.png?v=1781696421"},{"product_id":"bspei-restriction-enzyme-for-precision-dna-digestion","title":"BspEI Restriction Enzyme for Precision DNA Digestion","description":"BspEI is a Type IIP restriction enzyme derived from Bacillus and obtained by recombinant expression and purification in Escherichia coli. BspEI recognizes and cleaves the sequence TCCGGA, generating a 4-base 5' overhang. BspEI requires the dedicated Cut Buffer C and is incompatible with the universal CutOne® Buffer.","brand":"Cleavion","offers":[{"title":"500 U","offer_id":57188444930423,"sku":"CLV-0295","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0295-0-cleavion-product.png?v=1781696423"},{"product_id":"swai-restriction-enzyme-for-precision-dna-digestion","title":"SwaI Restriction Enzyme for Precision DNA Digestion","description":"","brand":"Cleavion","offers":[{"title":"1000 U","offer_id":57188445684087,"sku":"CLV-0300","price":98.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0300-0-cleavion-product.png?v=1781696426"},{"product_id":"esp3i-bsmbi-type-iis-restriction-enzyme-adcf","title":"Esp3I\/BsmBI Type IIS Restriction Enzyme, ADCF","description":"","brand":"Cleavion","offers":[{"title":"2000 U","offer_id":57188445880695,"sku":"CLV-0301","price":758.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0301-0-cleavion-product.png?v=1781696438"}],"url":"https:\/\/cleavion.com\/collections\/standard-specialty-restriction-enzymes.oembed?page=2","provider":"Cleavion Inc.","version":"1.0","type":"link"}