{"product_id":"t4-dna-ligase-fast","title":"T4 DNA Ligase (Fast)","description":"T4 DNA Ligase (Fast) is produced by E.coli carrying T4 bacteriophage gene 30. The enzyme catalyzes the formation of phosphodiester bonds between 5'-phosphate groups and 3'-hydroxyl groups of double-stranded DNA or RNA. It can repair single-strand nicks in double-stranded DNA, RNA, or DNA\/RNA hybrids and can ligate DNA fragments with sticky or blunt ends. It is not active on single-stranded nucleic acids. The enzyme is primarily used for cloning of restriction endonuclease-digested DNA fragments, site-directed mutagenesis, cloning of PCR products, linear DNA circularization, and repair of double-stranded DNA nicks. T4 DNA Ligase (Fast) requires ATP as a cofactor and completes sticky end ligation reactions at room temperature in just 10 minutes.","brand":"Cleavion","offers":[{"title":"1000 U","offer_id":57094766330231,"sku":"CLV-0162","price":165.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0162-0-cleavion.png?v=1778840201","url":"https:\/\/cleavion.com\/products\/t4-dna-ligase-fast","provider":"Cleavion","version":"1.0","type":"link"}