{"product_id":"t4-rna-ligase-2","title":"T4 RNA Ligase 1","description":"T4 RNA Ligase 1 is an ATP-dependent ligase derived from bacteriophage T4. It catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3 hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond with hydrolysis of ATP to AMP and PPi. The ligation efficiency of T4 RNA Ligase 1 varies significantly for different substrates, following the order: ssRNA-ssRNA\u0026gt;ssRNA-ssDNA\u0026gt;ssDNA-ssDNA. Additionally, T4 RNA Ligase 1 can also be used for ligation between RNA and mononucleotides, provided that the mononucleotides are phosphorylated at both the 5' and 3' ends. This application is commonly used for 3'-end labeling of RNA.","brand":"Cleavion","offers":[{"title":"5000 U","offer_id":57094769738103,"sku":"CLV-0198","price":482.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0991\/7097\/6119\/files\/CLV-0198-0-cleavion.png?v=1778840210","url":"https:\/\/cleavion.com\/products\/t4-rna-ligase-2","provider":"Cleavion","version":"1.0","type":"link"}