BbvCI Restriction Enzyme

BbvCI is derived from the BbvCI gene of Bacillus brevis and is obtained through recombinant expression in Escherichia coli. BbvCI is a Type IIA restriction enzyme that exists as a heterodimer composed of two distinct catalytic subunits, each with an independent cleavage site.

It recognizes and cleaves the non-palindromic sequence CCTCAGC (-5/-2), generating 5′ cohesive ends with 3-base overhangs. BbvCI uses the recommended reaction buffer and is compatible with double digestion with other rapid-digestion restriction enzymes. However, extended digestion exceeding 3 hours should be avoided to reduce the risk of star activity.

Restriction Endonucleases

Restriction Endonucleases

Recommended Reaction Conditions 1x CutOne® Buffer; Incubate at 37°C; Refer to "Protocol for DNA Digestion" for reaction setup. Heat Inactivation Incubation at 80°C for 20 minutes. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.

2 U/μL

CCTCAGC 5'...C C↓T C A G C...3' 3'...G G A G T↑C G ...5'

• BbvCI (2 U/μl): 25 μl
• 10× Universal Digestion Buffer: 1 ml
• 10× Color Loading Digestion Buffer: 1 ml

Store at -20°C for 2 years.

2~8°C