Cell-Free Protein Synthesis Kit (Lyophilized)

The Cell-Free Protein Synthesis Kit is a product for in vitro protein synthesis based on E. coli lysate. Coupled transcription/translation systems contain an RNA polymerase and the necessary cellular machinery needed to direct protein synthesis (e.g., ribosomes, translation factors and tRNAs) . Supplements such as amino acids, an energy source and NTPs complete the system. Proteins are expressed using DNA or RNA as templates. Cell-free protein expression, independent of viable cells, enables rapid, flexible and high-yield protein expression, with numerous advantages over traditional recombinant expression systems: 1. Time saving. Express target proteins in 1~2 hours, with peak yield achieved in 8~24 hours. 2. High protein expression level, up to more than 3 mg/ml. 3. Easy operation, Add DNA or RNA templates to the reaction system, and the reaction can be performed using 96-well plates or centrifuge tubes. Note: This product is not suitable for the soluble expression of disulfide-rich proteins, as it does not provide the oxidative environment required for correct disulfide bond folding. If needed, please contact us for other REF NO..

Protein Research

Cell-Free Protein Synthesis Kit

• Cell-free system solution A (Lyophilized) : 1 vial (1.5 ml after reconstitution) • Cell-free system solution B (Lyophilized) : 1 vial (3 ml after reconstitution) • CFPS-Control Plasmid: 2 μg

4°C transportation. Store at -20°C. Valid for 12 months. After reconstitution, store at -20°C for no more than 1 month, and at -80°C for no more than 12 months.

2~8°C