DNase I, RNase-Free (Lyophilized)

DNase I, RNase-free is an endodeoxyribonuclease that catalyzes to the same extent the degradation of both single- and double-stranded DNA randomly, and produces 5'-P terminal oligonucleotides. The activity of DNase I is dependent on Ca2+, and can be activated by bivalent metal ions Mg2+, Mn2+, etc. In the presence of Mg2+, the enzyme can randomly recognize and cut any site on any strand of double-stranded DNA. In the presence of Mn2+, nearly identical sites on the two strands of DNA can be recognized and cut, resulting in flat ends or sticky end DNA fragments with 1 to 2 nucleotides overhang. This product is provided in the form of lyophilized powder, which facilitates transportation and storage. However, once dissolved into a solution, please stored it at -20°C for long-term stability.

Modification and Cloning

Non-specific DNA Endonucleases & DNA Repair Enzymes

• DNase I, RNase-free (Lyophilized) : 1000 U • 10xDNase I Buffer: 1.25 ml

-20°C

2~8°C