HiFi Taq DNA Ligase

HiFi Taq DNA Ligase is a mutant type of Taq DNA ligase, which has higher ligation efficiency and high fidelity compared with the wild type. Meanwhile, it maintains the basic functions of the wild type and is a high-temperature-resistant ligase with activity in the range of 37~75°C .Taq DNA ligase, with NAD+ as the coenzyme factor, catalyzes the formation of a phosphodiester bond between the 5'-phosphate and 3'-hydroxyl groups of two adjacent oligonucleotide chains hybridized to the same complementary target DNA strand, and it has significant activity only for DNA nicks. This product is applicable to molecular diagnostic reactions that rely on nick junction, such as ligase detection reaction (LDR), multiplex ligation-dependent probe amplification (MLPA), etc.

Modification and Cloning

DNA Ligase

• HiFi Taq DNA Ligase: 50 rxns • 10x HiFi Taq DNA Ligase Buffer: 500 μl

-20°C

2~8°C