NruI Restriction Enzyme

Rapid-digestion restriction enzymes are engineered restriction enzymes designed for fast DNA digestion. These enzymes show strong activity in a universal digestion buffer and can digest DNA within 5–15 minutes. This enables different combinations of restriction enzymes to work simultaneously in a single reaction tube and reduces the need for sequential digestion steps.

Rapid-digestion restriction enzymes undergo strict quality control testing and can be used to digest plasmid DNA, genomic DNA, viral DNA, and PCR products. The color loading digestion buffer contains a density reagent together with red and yellow tracking dyes, allowing reaction mixtures to be loaded directly onto an agarose gel after digestion. In a 1% agarose gel, the red dye migrates similarly to approximately 2.5 kb double-stranded DNA fragments, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA fragments.

Restriction Endonucleases

Rapid-Digestion Restriction Endonucleases

The reaction can be completed within 15 minutes Recommended Reaction Conditions 1x CutOne® Buffer; Incubate at 37°C; Refer to "Protocol for Fast DNA Digestion" for reaction setup. Heat Inactivation This enzyme can not be heat inactivated. Please purify the digested product by phenol/chloroform treatment or column-based purification kit. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the Dam methylase. Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.

20 U/μL

TCGCGA 5'...T C G ↓ C G A...3' 3'...A G C ↑ G C T...5'

Bsp68I, BtuMI, RruI

• The reaction can be completed within **15 minutes**

**Recommended Reaction Conditions**
Use **1× Universal Digestion Buffer** and incubate at **37°C**. Refer to the **Fast DNA Digestion Protocol** for reaction setup.

**Heat Inactivation**
This enzyme **cannot be heat inactivated**. Purify the digested product using **phenol/chloroform extraction** or a **column-based purification kit**.

**Methylation Sensitivity**
Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by **Dam methylase**.

Cleavage with this restriction enzyme may also be blocked or impaired when the substrate DNA is methylated by **CpG methylase**.

-20°C

2~8°C