NsiI Restriction Enzyme

Rapid-digestion restriction enzymes are engineered restriction enzymes designed for fast DNA digestion. These enzymes show strong activity in a universal digestion buffer and can digest DNA within 5–15 minutes. This enables different combinations of restriction enzymes to work simultaneously in a single reaction tube and reduces the need for sequential digestion steps.

Rapid-digestion restriction enzymes undergo strict quality control testing and can be used to digest plasmid DNA, genomic DNA, viral DNA, and PCR products. The color loading digestion buffer contains a density reagent together with red and yellow tracking dyes, allowing reaction mixtures to be loaded directly onto an agarose gel after digestion. In a 1% agarose gel, the red dye migrates similarly to approximately 2.5 kb double-stranded DNA fragments, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA fragments.

Restriction Endonucleases

Rapid-Digestion Restriction Endonucleases

The reaction can be completed within 15 minutes Recommended Reaction Conditions 1x CutOne® Buffer; Incubate at 37°C; Refer to "Protocol for Fast DNA Digestion" for reaction setup. Heat Inactivation Incubation at 80°C for 20 minutes. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the EcoBI methylase.

20 U/μL

ATGCAT 5'...A T G C A ↓ T...3' 3'...T ↑ A C G T A...5'

EcoT22I, Mph1103I, Zsp2I

Recommended Reaction Conditions
1× Universal Digestion Buffer; incubate at 37°C. Refer to the Fast DNA Digestion Protocol for reaction setup.

Heat Inactivation
Incubate at 80°C for 20 minutes.

Methylation Sensitivity
Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by EcoBI methylase.

-20°C

2~8°C