• PNGase F (with His-Tag)
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PNGase F (with His-Tag)

PNGase F (with His-Tag)

CLV-0219

$444.88 USD
$292.50 USD $444.88 USD
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Product Specifications

  • Description

    PNGase F (Peptide N-Glycosidase F) is a high effective amidase derived from Elizabethkingia miricola . It cleaves asparagine-linked high mannose as well as hybrid and complex oligosaccharides from glycoproteins. The cleavage site of PNGase F is the amide bond between the N-acetylglucosamine (GlcNAc) inside the protein and the asparagine residue, while converting the asparagine residue in the digested protein to aspartic acid. This product is expressed through recombinant expression in E.coli , and it contains a His tag and 50% glycerol. It is commonly used for deglycosylation of antibodies and related proteins.

  • Category

    Protein Research

  • Sub Category

    Proteinase

  • Concentration

    500 U/μL

  • Components

    • PNGase F (500 U/μl) : 150 μl • 10x Denaturing Buffer: 750 μl • 10x PNGase F Buffer: 1 ml • 10% NP-40: 1 ml

  • Storage Conditions

    -20°C

  • Shipping Conditions

    2~8°C

PNGase F (with His-Tag)

PNGase F (Peptide N-Glycosidase F) is a high effective amidase derived from Elizabethkingia miricola . It cleaves asparagine-linked high mannose as well as hybrid and complex oligosaccharides from glycoproteins. The cleavage site of PNGase F is the amide bond between the N-acetylglucosamine (GlcNAc) inside the protein and the asparagine residue, while converting the asparagine residue in the digested protein to aspartic acid. This product is expressed through recombinant expression in E.coli , and it contains a His tag and 50% glycerol. It is commonly used for deglycosylation of antibodies and related proteins.