SapI Restriction Enzyme

LightNing® enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All LightNing® enzymes show superior activity in the universal CutOne® and CutOne® Color Buffer, and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. LightNing® enzymes have passed multiple strict quality controls, and can be used to digest plasmid, genomic and viral DNA as well as PCR products. CutOne® Color Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the CutOne® Color Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.

Restriction Endonucleases

LightNing® Restriction Endonucleases

The reaction can be completed within 15 minutes Recommended Reaction Conditions 1x CutOne® Buffer; Incubate at 37°C; Refer to "Protocol for Fast DNA Digestion" for reaction setup. Heat Inactivation Incubation at 80°C for 20 minutes. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the EcoBI methylase.

10 U/μL

GCTCTTC (1/4) 5'...G C T C T T C (N) 1↓...3' 3'...C G A G A A G (N) 4↑...5'

BspQI, LguI, PciSI

• LightNing® SapI (10 U/μl) : 50 μl (500 U) • 10x CutOne® Buffer: 1 ml • 10x CutOne® Color Buffer: 1 ml

Store at -20°C for 2 years.

2~8°C