• SpCas9-NLS
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SpCas9-NLS

SpCas9-NLS

CLV-0192

$252.50 USD
$150.00 USD $252.50 USD
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Product Specifications

  • Description

    SpCas9-NLS is an RNA-guided endonuclease from Streptococcus pyogenes. Guided by crRNA and tracrRNA (or a sgRNA formed by their fusion), SpCas9-NLS recognizes the region immediately upstream of the PAM sequence (5'-NGG) in double-stranded DNA targets and produces a double-stranded break precisely three nucleotides upstream of the PAM. SpCas9-NLS is commonly employed for gene editing. To enhance cellular editing efficiency, SpCas9-NLS contains a nuclear localization sequence (NLS) derived from Simian virus 40 (SV40) T antigen on N-termini. Additionally, this product can be utilized for the cleavage of target DNA in vitro and gene cloning, etc.

  • Category

    Modification and Cloning

  • Sub Category

    Gene Editing Enzymes

  • Concentration

    10 μM

  • Components

    • SpCas9-NLS (10 μM) : 100 μl • 10x Cut Buffer C: 1 ml

  • Storage Conditions

    Store at -20°C for 2 years.

  • Shipping Conditions

    2~8°C

SpCas9-NLS

SpCas9-NLS is an RNA-guided endonuclease from Streptococcus pyogenes. Guided by crRNA and tracrRNA (or a sgRNA formed by their fusion), SpCas9-NLS recognizes the region immediately upstream of the PAM sequence (5'-NGG) in double-stranded DNA targets and produces a double-stranded break precisely three nucleotides upstream of the PAM. SpCas9-NLS is commonly employed for gene editing. To enhance cellular editing efficiency, SpCas9-NLS contains a nuclear localization sequence (NLS) derived from Simian virus 40 (SV40) T antigen on N-termini. Additionally, this product can be utilized for the cleavage of target DNA in vitro and gene cloning, etc.