• T5 Exonuclease
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T5 Exonuclease

T5 Exonuclease

CLV-0178

$89.43 USD
$34.29 USD $89.43 USD
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Product Specifications

  • Description

    T5 Exonuclease is a nucleic acid exonuclease that digests DNA from the 5' end in the 5'→3' direction for both double-stranded and single-stranded DNA. It is capable of digesting DNA at nicks or gaps in linear or circular double-stranded DNA, however, it cannot digest supercoiled DNA. In addition, when the Mg2+ concentration in the solution is below 1 mM, the activity of T5 Exonuclease towards single-stranded DNA is inhibited. Consequently, T5 Exonuclease is particularly suitable for degrading linearized and nicked plasmids, removing non-circular DNA from ligation products, seamless cloning (Gibson Assembly), and other applications.

  • Category

    Modification and Cloning

  • Sub Category

    DNA Exonucleases

  • Concentration

    10 U/μL

  • Components

    • T5 Exonuclease (10 U/μl) : 100 μl • 10x Cut Buffer D: 1 ml

  • Storage Conditions

    -20°C

  • Shipping Conditions

    2~8°C

T5 Exonuclease

T5 Exonuclease is a nucleic acid exonuclease that digests DNA from the 5' end in the 5'→3' direction for both double-stranded and single-stranded DNA. It is capable of digesting DNA at nicks or gaps in linear or circular double-stranded DNA, however, it cannot digest supercoiled DNA. In addition, when the Mg2+ concentration in the solution is below 1 mM, the activity of T5 Exonuclease towards single-stranded DNA is inhibited. Consequently, T5 Exonuclease is particularly suitable for degrading linearized and nicked plasmids, removing non-circular DNA from ligation products, seamless cloning (Gibson Assembly), and other applications.