T7 High Yield RNA Synthesis Kit

The T7 High Yield RNA Synthesis Kit is designed for the large-scale synthesis of RNA through in vitro transcription using T7 RNA polymerase and DNA templates containing a T7 promoter. It is suitable for both long and short RNA transcripts. The T7 Enzyme Mix included RNase inhibitor and inorganic pyrophosphatase. Additionally, DNase I, RNase-free, is provided to eliminate template DNA after the transcription reaction. Using this kit, 1 μg of linearized double-stranded DNA template can yield at least 150 μg of RNA. The synthesized RNA is applicable for a wide range of downstream applications, including in vitro translation, RNA structure and function studies, RNase protection assays, probe hybridization, RNA interference, and more.

In Vitro Transcription

In Vitro Transcription

• T7 Enzyme Mix: 100 μl • 10x T7 RNA Pol Buffer: 100 μl • ATP (100mM) : 100 μl • UTP (100mM) : 100 μl • GTP (100mM) : 100 μl • CTP (100mM) : 100 μl • Control Template (0.5 μg/μl) : 10 μl • DNase I, RNase Free (1 U/μl) : 100 μl • Nuclease-Free Water: 1 ml

-20°C

2~8°C