XhoI Restriction Enzyme

Rapid-digestion restriction enzymes are engineered restriction enzymes designed for fast DNA digestion. These enzymes show strong activity in a universal digestion buffer and can digest DNA within 5–15 minutes. This enables different combinations of restriction enzymes to work simultaneously in a single reaction tube and reduces the need for sequential digestion steps.

Rapid-digestion restriction enzymes undergo strict quality control testing and can be used to digest plasmid DNA, genomic DNA, viral DNA, and PCR products. The color loading digestion buffer contains a density reagent together with red and yellow tracking dyes, allowing reaction mixtures to be loaded directly onto an agarose gel after digestion. In a 1% agarose gel, the red dye migrates similarly to approximately 2.5 kb double-stranded DNA fragments, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA fragments.

Restriction Endonucleases

Rapid-Digestion Restriction Endonucleases

The reaction can be completed within 15 minutes Recommended Reaction Conditions 1x CutOne® Buffer; Incubate at 37°C; Refer to "Protocol for Fast DNA Digestion" for reaction setup. Heat Inactivation Incubation at 80°C for 20 minutes. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.

20 U/μL

CTCGAG 5'...C ↓ T C G A G...3' 3'...G A G C T ↑ C...5'

PaeR7I, Sfr274I, SlaI

• Rapid-digestion XhoI (20 U/μl): 500 μl / 10,000 U
• 10× Universal Digestion Buffer: 2 × 1 ml
• 10× Color Loading Digestion Buffer: 2 × 1 ml

-20°C

2~8°C